BACKGROUND: The impact of industrial trans fatty acids (TFAs) on lipid metabolism and health remains elusive. METHODS: We compared the effect of long-term (52 weeks) ingestion of 10% partially hydrogenated vegetable oil, providing 4.2% of total energy from TFAs, on hepatic lipid metabolism and muscle insulin sensitivity in weanling female Fischer rats with that of palmolein (monounsaturated fatty acid, MUFA), sunflower (n-6 polyunsaturated fatty acid, PUFA), and a blend of sunflower and fish oil (n-3 PUFA). RESULTS: The proportion of plasma high-density lipoprotein cholesterol in total cholesterol and reverse cholesterol transport-associated protein expressions were similar in all the groups. Despite higher lipogenic-pathway protein levels, steatosis or hypertriglyceridemia was not observed in TFA-fed rats. Though TFA ingestion had no effect on fasting plasma glucose, insulin levels or oral glucose tolerance, it significantly decreased muscle insulin-stimulated glucose uptake as compared to PUFAs. Further, TFA ingestion increased adipose tissue retinol-binding protein 4 mRNA as compared to PUFAs (n-6 or n-3). The effects of MUFA (oleic acid) on all these parameters were comparable to those observed for TFAs. CONCLUSIONS: Compared to PUFA-rich diets, chronic consumption of a TFA-rich diet did not lead to steatosis or hypertriglyceridemia; however, it significantly impaired muscle insulin sensitivity, while no changes were found in the oral glucose tolerance test.
BACKGROUND: The impact of industrial trans fatty acids (TFAs) on lipid metabolism and health remains elusive. METHODS: We compared the effect of long-term (52 weeks) ingestion of 10% partially hydrogenated vegetable oil, providing 4.2% of total energy from TFAs, on hepatic lipid metabolism and muscle insulin sensitivity in weanling female Fischer rats with that of palmolein (monounsaturated fatty acid, MUFA), sunflower (n-6 polyunsaturated fatty acid, PUFA), and a blend of sunflower and fish oil (n-3 PUFA). RESULTS: The proportion of plasma high-density lipoprotein cholesterol in total cholesterol and reverse cholesterol transport-associated protein expressions were similar in all the groups. Despite higher lipogenic-pathway protein levels, steatosis or hypertriglyceridemia was not observed in TFA-fed rats. Though TFA ingestion had no effect on fasting plasma glucose, insulin levels or oral glucose tolerance, it significantly decreased muscle insulin-stimulated glucose uptake as compared to PUFAs. Further, TFA ingestion increased adipose tissue retinol-binding protein 4 mRNA as compared to PUFAs (n-6 or n-3). The effects of MUFA (oleic acid) on all these parameters were comparable to those observed for TFAs. CONCLUSIONS: Compared to PUFA-rich diets, chronic consumption of a TFA-rich diet did not lead to steatosis or hypertriglyceridemia; however, it significantly impaired muscle insulin sensitivity, while no changes were found in the oral glucose tolerance test.
Authors: Heidi T M Lai; Fumiaki Imamura; Andres V Ardisson Korat; Rachel A Murphy; Nathan Tintle; Julie K Bassett; Jiaying Chen; Janine Kröger; Kuo-Liong Chien; Mackenzie Senn; Alexis C Wood; Nita G Forouhi; Matthias B Schulze; William S Harris; Ramachandran S Vasan; Frank Hu; Graham G Giles; Allison Hodge; Luc Djousse; Ingeborg A Brouwer; Frank Qian; Qi Sun; Jason H Y Wu; Matti Marklund; Rozenn N Lemaitre; David S Siscovick; Amanda M Fretts; Aladdin H Shadyab; JoAnn E Manson; Barbara V Howard; Jennifer G Robinson; Robert B Wallace; Nick J Wareham; Yii-Der Ida Chen; Jerome I Rotter; Michael Y Tsai; Renata Micha; Dariush Mozaffarian Journal: Diabetes Care Date: 2022-04-01 Impact factor: 17.152
Authors: Débora Estadella; Claudia M da Penha Oller do Nascimento; Lila M Oyama; Eliane B Ribeiro; Ana R Dâmaso; Aline de Piano Journal: Mediators Inflamm Date: 2013-01-31 Impact factor: 4.711