OBJECTIVE: To study the characteristics of lymphocyte nuclear factor kappa B (NF-κB) signal transduction kinase-related molecular mRNA differential expressions at various month age segments in aging process and the intervening effect of Epimedium flavonoids (EF) on it. METHODS: Sixty SD rats were divided into six groups, according to animals' age, i.e., the 3 days (d) group, the 4 months (m) group, the 10 m group, the 18 m group, the 27 m group, and the 27 m+EF group. RNA was extracted from separated splenic lymphocytes. Adopting NF-κB signal path functional genome oligonucleotide gene-chip (128 related genes), the integral characteristics and differences of NF-κB signal transduction kinase-related mRNA expressions were determined, and the intervening effect of EF was examined. RESULTS: The mean level of the NF-κB signal transduction kinase-related mRNA expressions in rats' splenic lymphocytes lowered with aging; the highest expression was presented at 3 d after birth, and then, it lowered gradually, with the lowest level at 18 m or 27 m. After EF intervention, the expression level was raised to the 10-18 m level in the aged rats. CONCLUSION: The changing rules of lymphocyte NF-κB-signal-transduction-kinase-related mRNA expressions in various stages of aging are helpful for selecting the well time for preventing and intervening aging, and will also give a hint to the molecular index for assessment of senility retarding researches.
OBJECTIVE: To study the characteristics of lymphocyte nuclear factor kappa B (NF-κB) signal transduction kinase-related molecular mRNA differential expressions at various month age segments in aging process and the intervening effect of Epimedium flavonoids (EF) on it. METHODS: Sixty SD rats were divided into six groups, according to animals' age, i.e., the 3 days (d) group, the 4 months (m) group, the 10 m group, the 18 m group, the 27 m group, and the 27 m+EF group. RNA was extracted from separated splenic lymphocytes. Adopting NF-κB signal path functional genome oligonucleotide gene-chip (128 related genes), the integral characteristics and differences of NF-κB signal transduction kinase-related mRNA expressions were determined, and the intervening effect of EF was examined. RESULTS: The mean level of the NF-κB signal transduction kinase-related mRNA expressions in rats' splenic lymphocytes lowered with aging; the highest expression was presented at 3 d after birth, and then, it lowered gradually, with the lowest level at 18 m or 27 m. After EF intervention, the expression level was raised to the 10-18 m level in the aged rats. CONCLUSION: The changing rules of lymphocyte NF-κB-signal-transduction-kinase-related mRNA expressions in various stages of aging are helpful for selecting the well time for preventing and intervening aging, and will also give a hint to the molecular index for assessment of senility retarding researches.
Authors: Mi Kyung Kim; Sang Woon Chung; Dae Hyun Kim; Ji Min Kim; Eun Kyeong Lee; Ji Young Kim; Young Mi Ha; Yun Hee Kim; Jae-Kyung No; Hye Sun Chung; Kun-Young Park; Sook Hee Rhee; Jae Sue Choi; Byung Pal Yu; Takako Yokozawa; Young Jin Kim; Hae Young Chung Journal: Exp Gerontol Date: 2010-03-06 Impact factor: 4.032
Authors: Xiang Li; Paul E Massa; Adedayo Hanidu; Gregory W Peet; Patrick Aro; Ann Savitt; Sheenah Mische; Jun Li; Kenneth B Marcu Journal: J Biol Chem Date: 2002-09-06 Impact factor: 5.157
Authors: Stephen C Bondy; Huihui Li; Jun Zhou; Meixia Wu; Jason A Bailey; Debomoy K Lahiri Journal: Neurochem Res Date: 2010-06-10 Impact factor: 3.996