OBJECTIVE: To investigate the cellular effects of Pien Tze Huang (PZH) in the HT-29 human colon carcinoma cell line. METHODS: The viability of HT-29 cells was determined by MTT assay. A fluorescence-activated cell sorting (FACS) analysis with annexin-V/propidium iodide (PI) and JC-1 staining were performed to determine cell apoptosis and the loss of mitochondrial membrane potential, respectively. Activation of caspase 3 was evaluated by a colorimetric assay. The mRNA expression levels of Bcl-2 and Bax were measured by reverse transcription polymerase chain reaction (RT-PCR). RESULTS: PZH, in a dose- and time-dependent manner, reduced viability and induced apoptosis of HT-29 cells. Moreover, PZH treatment resulted in the collapse of the mitochondrial membrane potential, activation of caspase 3, and an increase in the Bax/Bcl-2 ratio. CONCLUSION: PZH inhibits the growth of HT-29 cells by inducing cancer cell apoptosis via regulation of the Bcl-2 family and activation of caspase 3, which may, in part, explain its anticancer activity.
OBJECTIVE: To investigate the cellular effects of Pien Tze Huang (PZH) in the HT-29 humancolon carcinoma cell line. METHODS: The viability of HT-29 cells was determined by MTT assay. A fluorescence-activated cell sorting (FACS) analysis with annexin-V/propidium iodide (PI) and JC-1 staining were performed to determine cell apoptosis and the loss of mitochondrial membrane potential, respectively. Activation of caspase 3 was evaluated by a colorimetric assay. The mRNA expression levels of Bcl-2 and Bax were measured by reverse transcription polymerase chain reaction (RT-PCR). RESULTS: PZH, in a dose- and time-dependent manner, reduced viability and induced apoptosis of HT-29 cells. Moreover, PZH treatment resulted in the collapse of the mitochondrial membrane potential, activation of caspase 3, and an increase in the Bax/Bcl-2 ratio. CONCLUSION: PZH inhibits the growth of HT-29 cells by inducing cancer cell apoptosis via regulation of the Bcl-2 family and activation of caspase 3, which may, in part, explain its anticancer activity.
Authors: Jun Peng; Chibing Tan; G Jane Roberts; Olga Nikolaeva; Zhi Zhang; Suzanne M Lapolla; Steve Primorac; David W Andrews; Jialing Lin Journal: J Biol Chem Date: 2006-09-27 Impact factor: 5.157
Authors: B Antonsson; F Conti; A Ciavatta; S Montessuit; S Lewis; I Martinou; L Bernasconi; A Bernard; J J Mermod; G Mazzei; K Maundrell; F Gambale; R Sadoul; J C Martinou Journal: Science Date: 1997-07-18 Impact factor: 47.728