Literature DB >> 2190950

Detection of DNA sequences in Plasmodium berghei by means of in situ hybridization.

M Obst1, A W Cornelissen.   

Abstract

A non-radioactive in situ hybridization technique, used to map unique DNA sequences to plant chromosomes, has been adapted for the localization of specific DNA sequences in nuclei of Plasmodium berghei. After hybridization using probes labeled with biotin-11-dUTP, the formed DNA/DNA hybrids were detected by fluorescence microscopy using a specific double-layer antibody technique. Besides its high resolution, this procedure is characterized by a high sensitivity, allowing the detection of a unique sequence as small as 2.5 kb.

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Year:  1990        PMID： 2190950     DOI： 10.1007/bf00266796

Source DB:  PubMed          Journal:  Histochemistry        ISSN： 0301-5564


  32 in total

1.  Identification, purification and properties of clone-specific glycoprotein antigens constituting the surface coat of Trypanosoma brucei.

Authors:  G A Cross
Journal:  Parasitology       Date:  1975-12       Impact factor: 3.234

2.  Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors:  P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal:  J Mol Biol       Date:  1977-06-15       Impact factor: 5.469

Review 3.  Sex determination and sex differentiation in malaria parasites.

Authors:  A W Cornelissen
Journal:  Biol Rev Camb Philos Soc       Date:  1988-08

Review 4.  Sexual processes in parasitic protozoa.

Authors:  J R Baker
Journal:  Int J Parasitol       Date:  1989-08       Impact factor: 3.981

5.  Trypanosoma brucei contains two RNA polymerase II largest subunit genes with an altered C-terminal domain.

Authors:  R Evers; A Hammer; J Köck; W Jess; P Borst; S Mémet; A W Cornelissen
Journal:  Cell       Date:  1989-02-24       Impact factor: 41.582

6.  Ribosomal DNA sequences detected in malaria parasites by cytochemical hybridization.

Authors:  A W Cornelissen; G Langsley; D Walliker
Journal:  Biol Cell       Date:  1983       Impact factor: 4.458

7.  Chromosomal localization of a unique gene by non-autoradiographic in situ hybridization.

Authors:  J E Landegent; N Jansen in de Wal; G J van Ommen; F Baas; J J de Vijlder; P van Duijn; M Van der Ploeg
Journal:  Nature       Date:  1985 Sep 12-18       Impact factor: 49.962

8.  Gametocytogenesis and ribosomal rRNA gene organisation in the rodent malarias Plasmodium chabaudi and Plasmodium berghei.

Authors:  A W Cornelissen; G Langsley; D Walliker; J G Scaife
Journal:  Mol Biochem Parasitol       Date:  1985-02       Impact factor: 1.759

9.  Determination of nuclear DNA of five eucoccidian parasites, Isospora (Toxoplasma) gondii, Sarcocystis cruzi, Eimeria tenella, E. acervulina and Plasmodium berghei, with special reference to gamontogenesis and meiosis in I. (T.) gondii.

Authors:  A W Cornelissen; J P Overdulve; M van der Ploeg
Journal:  Parasitology       Date:  1984-06       Impact factor: 3.234

10.  Cytochemical studies on nuclear DNA of four eucoccidian parasites, Isospora (Toxoplasma) gondii, Eimeria tenella, Sarcocystis cruzi and Plasmodium berghei.

Authors:  A W Cornelissen; J P Overdulve; M Van der Ploeg
Journal:  Parasitology       Date:  1984-02       Impact factor: 3.234
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