OBJECTIVES: We investigated whether Abatacept might reduce proinflammatory cytokine production by macrophages upon contact with cytokine activated T cells and/or stimulation with TLR ligands. METHODS: Macrophages and cytokine stimulated T cells (Tck) were added together in the presence of Abatacept or a control Ig, with or without TLR ligands. The production of cytokines was determined by luminex. RESULTS: Abatacept reduced Tck-induced production of TNFa by macrophages. Tck and TLR ligands synergistically induced the production of proinflammatory cytokines by macrophages, especially IL-12p70. The production of IL-12p70 coincided with the production of IFNg, which were both reduced in the presence of Abatacept. CONCLUSIONS: Tck induce the production of TNFa by macrophages and facilitate the highly increased production of proinflammatory cytokines in the presence of TLR ligands. Abatacept was shown to potently suppress these pathways suggesting that its role may extend beyond antigen specific T cell mediated effector function.
OBJECTIVES: We investigated whether Abatacept might reduce proinflammatory cytokine production by macrophages upon contact with cytokine activated T cells and/or stimulation with TLR ligands. METHODS: Macrophages and cytokine stimulated T cells (Tck) were added together in the presence of Abatacept or a control Ig, with or without TLR ligands. The production of cytokines was determined by luminex. RESULTS: Abatacept reduced Tck-induced production of TNFa by macrophages. Tck and TLR ligands synergistically induced the production of proinflammatory cytokines by macrophages, especially IL-12p70. The production of IL-12p70 coincided with the production of IFNg, which were both reduced in the presence of Abatacept. CONCLUSIONS: Tck induce the production of TNFa by macrophages and facilitate the highly increased production of proinflammatory cytokines in the presence of TLR ligands. Abatacept was shown to potently suppress these pathways suggesting that its role may extend beyond antigen specific T cell mediated effector function.
Authors: Iris Hecht; Amir Toporik; Joseph R Podojil; Ilan Vaknin; Gady Cojocaru; Anat Oren; Elizabeta Aizman; Spencer C Liang; Ling Leung; Yosef Dicken; Amit Novik; Nadav Marbach-Bar; Aziza Elmesmari; Clare Tange; Ashley Gilmour; Donna McIntyre; Mariola Kurowska-Stolarska; Kay McNamee; Judith Leitner; Shirley Greenwald; Liat Dassa; Zurit Levine; Peter Steinberger; Richard O Williams; Stephen D Miller; Iain B McInnes; Eyal Neria; Galit Rotman Journal: J Immunol Date: 2018-02-05 Impact factor: 5.422
Authors: Benjamin J Kopecky; Christian Frye; Yuriko Terada; Keki R Balsara; Daniel Kreisel; Kory J Lavine Journal: Am J Transplant Date: 2020-01-29 Impact factor: 8.086
Authors: Diahann T S L Jansen; Hanane el Bannoudi; Ramon Arens; Kim L L Habets; Marjolijn Hameetman; Tom W J Huizinga; Jeroen N Stoop; René E M Toes Journal: Arthritis Res Ther Date: 2015-08-20 Impact factor: 5.156
Authors: Thierry P P van den Bosch; Nynke M Kannegieter; Dennis A Hesselink; Carla C Baan; Ajda T Rowshani Journal: Front Immunol Date: 2017-02-16 Impact factor: 7.561