OBJECTIVE: To measure the circulating concentrations of nitric oxide (NO) adducts with NO bioactivity after inhaled NO (iNO) therapy in infants with pulmonary hypertension. STUDY DESIGN: In this single center study, 5 sequential blood samples were collected from infants with pulmonary hypertension before, during, and after therapy with iNO (n = 17). Samples were collected from a control group of hospitalized infants without pulmonary hypertension (n = 16) and from healthy adults for comparison (n = 12). RESULTS: After beginning iNO (20 ppm) whole blood nitrite levels increased approximately two-fold within 2 hours (P<.01). Whole blood nitrate levels increased to 4-fold higher than baseline during treatment with 20 ppm iNO (P<.01). S-nitrosohemoglobin increased measurably after beginning iNO (P<.01), whereas iron nitrosyl hemoglobin and total hemoglobin-bound NO-species compounds did not change. CONCLUSION: Treatment of pulmonary hypertensive infants with iNO results in increases in levels of nitrite, nitrate, and S-nitrosohemoglobin in circulating blood. We speculate that these compounds may be carriers of NO bioactivity throughout the body and account for peripheral effects of iNO in the brain, heart, and other organs.
OBJECTIVE: To measure the circulating concentrations of nitric oxide (NO) adducts with NO bioactivity after inhaled NO (iNO) therapy in infants with pulmonary hypertension. STUDY DESIGN: In this single center study, 5 sequential blood samples were collected from infants with pulmonary hypertension before, during, and after therapy with iNO (n = 17). Samples were collected from a control group of hospitalized infants without pulmonary hypertension (n = 16) and from healthy adults for comparison (n = 12). RESULTS: After beginning iNO (20 ppm) whole blood nitrite levels increased approximately two-fold within 2 hours (P<.01). Whole blood nitrate levels increased to 4-fold higher than baseline during treatment with 20 ppm iNO (P<.01). S-nitrosohemoglobin increased measurably after beginning iNO (P<.01), whereas iron nitrosyl hemoglobin and total hemoglobin-bound NO-species compounds did not change. CONCLUSION: Treatment of pulmonary hypertensiveinfants with iNO results in increases in levels of nitrite, nitrate, and S-nitrosohemoglobin in circulating blood. We speculate that these compounds may be carriers of NO bioactivity throughout the body and account for peripheral effects of iNO in the brain, heart, and other organs.
Authors: D L Pearson; S Dawling; W F Walsh; J L Haines; B W Christman; A Bazyk; N Scott; M L Summar Journal: N Engl J Med Date: 2001-06-14 Impact factor: 91.245
Authors: M T Gladwin; F P Ognibene; L K Pannell; J S Nichols; M E Pease-Fye; J H Shelhamer; A N Schechter Journal: Proc Natl Acad Sci U S A Date: 2000-08-29 Impact factor: 11.205
Authors: R O Cannon; A N Schechter; J A Panza; F P Ognibene; M E Pease-Fye; M A Waclawiw; J H Shelhamer; M T Gladwin Journal: J Clin Invest Date: 2001-07 Impact factor: 14.808
Authors: Anni Vanhatalo; Stephen J Bailey; Jamie R Blackwell; Fred J DiMenna; Toby G Pavey; Daryl P Wilkerson; Nigel Benjamin; Paul G Winyard; Andrew M Jones Journal: Am J Physiol Regul Integr Comp Physiol Date: 2010-08-11 Impact factor: 3.619
Authors: R Nevière; B Guery; S Mordon; F Zerimech; S Charré; F Wattel; C Chopin Journal: Am J Physiol Heart Circ Physiol Date: 2000-06 Impact factor: 4.733
Authors: Timothy J McMahon; Richard E Moon; Ben P Luschinger; Martha S Carraway; Anne E Stone; Bryant W Stolp; Andrew J Gow; John R Pawloski; Paula Watke; David J Singel; Claude A Piantadosi; Jonathan S Stamler Journal: Nat Med Date: 2002-06-03 Impact factor: 53.440
Authors: Mark T Gladwin; Xunde Wang; Christopher D Reiter; Benjamin K Yang; Esther X Vivas; Celia Bonaventura; Alan N Schechter Journal: J Biol Chem Date: 2002-05-21 Impact factor: 5.157
Authors: Jesica A Kanady; A Wilson Aruni; Janet R Ninnis; Andrew O Hopper; Jamie D Blood; Benjamin L Byrd; Leighton R Holley; Michael R Staker; Shandee Hutson; Hansel M Fletcher; Gordon G Power; Arlin B Blood Journal: Nitric Oxide Date: 2012-07-26 Impact factor: 4.427
Authors: Philip L Ballard; Roberta L Keller; Dennis M Black; David J Durand; Jeffrey D Merrill; Eric C Eichenwald; William E Truog; Mark C Mammel; Robin Steinhorn; Rita M Ryan; Sherry E Courtney; Hart Horneman; Roberta A Ballard Journal: Am J Perinatol Date: 2014-06-26 Impact factor: 1.862
Authors: Jesica A Jones; Janet R Ninnis; Andrew O Hopper; Yomna Ibrahim; T Allen Merritt; Kim-Wah Wan; Gordon G Power; Arlin B Blood Journal: JPEN J Parenter Enteral Nutr Date: 2013-07-26 Impact factor: 4.016
Authors: Jochen Gille; Hans-Jürgen Seyfarth; Stefan Gerlach; Michael Malcharek; Elke Czeslick; Armin Sablotzki Journal: Anesthesiol Res Pract Date: 2012-10-12