Qiaozhen Xie1, Wei Gao, Jifeng Li, Ting Qiao, Jing Jin, Huihua Deng, Zuhong Lu. 1. Key Laboratory of Child Development and Learning Science (Southeast University), Ministry of Education, Research Center of Learning Science, Southeast University, Nanjing, PR China.
Abstract
BACKGROUND: Cortisol level in human hair would be an endogenous biomarker for the retrospective assessment of long-term central hypothalamo-pituitary-adrenal activity. However, no direct evidence supports that blood-related diffusion is a biologically endogenous source of hair cortisol in humans. The present study aims to validate the direct correlation between cortisol in 1-cm hair segments and salivary cortisol in healthy humans. METHODS: We collected three saliva samples from the same participant at Time 1, Time 2 (1 week later) and Time 3 (2 weeks later), and hair 4 weeks later. Cortisol levels in 1-cm hair segments and saliva were determined with high performance liquid chromatography tandem mass spectrometry. RESULTS: Salivary cortisol at Time 1 was significantly associated with that at Time 2 (r=0.514, p=0.003), but not with that at Time 3 (r=0.187, p=0.305); and the one at Time 2 was significantly associated with that at Time 3 (r=0.380, p=0.032). Hair cortisol was significantly correlated with salivary cortisol at Time 2 (r=0.389, p<0.05) and average salivary cortisol (r=0.383, p<0.05) from three sampling. CONCLUSIONS: Our results confirmed that blood-related diffusion mechanism is a biologically endogenous source of hair cortisol.
BACKGROUND:Cortisol level in human hair would be an endogenous biomarker for the retrospective assessment of long-term central hypothalamo-pituitary-adrenal activity. However, no direct evidence supports that blood-related diffusion is a biologically endogenous source of hair cortisol in humans. The present study aims to validate the direct correlation between cortisol in 1-cm hair segments and salivary cortisol in healthy humans. METHODS: We collected three saliva samples from the same participant at Time 1, Time 2 (1 week later) and Time 3 (2 weeks later), and hair 4 weeks later. Cortisol levels in 1-cm hair segments and saliva were determined with high performance liquid chromatography tandem mass spectrometry. RESULTS: Salivary cortisol at Time 1 was significantly associated with that at Time 2 (r=0.514, p=0.003), but not with that at Time 3 (r=0.187, p=0.305); and the one at Time 2 was significantly associated with that at Time 3 (r=0.380, p=0.032). Hair cortisol was significantly correlated with salivary cortisol at Time 2 (r=0.389, p<0.05) and average salivary cortisol (r=0.383, p<0.05) from three sampling. CONCLUSIONS: Our results confirmed that blood-related diffusion mechanism is a biologically endogenous source of hair cortisol.
Authors: E M Tucker-Drob; A D Grotzinger; D A Briley; L E Engelhardt; F D Mann; M Patterson; C Kirschbaum; E K Adam; J A Church; J L Tackett; K P Harden Journal: Psychol Med Date: 2017-01-19 Impact factor: 7.723
Authors: Adaeze C Wosu; Unnur Valdimarsdóttir; Alexandra E Shields; David R Williams; Michelle A Williams Journal: Ann Epidemiol Date: 2013-10-05 Impact factor: 3.797