BACKGROUND: Chronic rhinosinusitis (CRS) is a heterogeneous disease characterized by local inflammation of the upper airways and sinuses and is frequently divided into polypoid CRS (CRSwNP) and nonpolypoid CRS (CRSsNP). However, the mechanism of inflammation in CRS has still not been fully elucidated. The aim of the study was to investigate the role of interleukin-32 (IL-32), a recently discovered proinflammatory cytokine, in CRS. METHODS: We collected nasal epithelial cells and nasal tissue from patients with CRS and control subjects. We assayed mRNA for IL-32 by real-time PCR and measured IL-32 protein using ELISA, Western blot, and immunohistochemistry. RESULTS: The expression of mRNA for IL-32 was elevated in epithelial cells from uncinate tissue from patients with CRSsNP compared with patients with CRSwNP (P < 0.05), control subjects (P=0.06), and epithelial cells from nasal polyp (NP) tissue (P < 0.05). Production of IL-32 was induced by IFN-γ, TNF, and dsRNA in primary airway epithelial cells. In whole-tissue extracts, the expression of IL-32 protein was significantly elevated in patients with CRSwNP compared with patients with CRSsNP and control subjects. Immunohistochemistry data showed that IL-32 was detected in mucosal epithelial cells and inflammatory cells in the lamina propria. Levels of IL-32 were correlated with the levels of CD3 and macrophage mannose receptor in NP tissue. Immunofluorescence data showed IL-32 co-localization with CD3-positive T cells and CD68-positive macrophages in NPs. CONCLUSION: Overproduction of IL-32 may be involved in the pathogenesis of CRS, although the role of IL-32 in the inflammation in CRSsNP and CRSwNP may be different.
BACKGROUND:Chronic rhinosinusitis (CRS) is a heterogeneous disease characterized by local inflammation of the upper airways and sinuses and is frequently divided into polypoid CRS (CRSwNP) and nonpolypoid CRS (CRSsNP). However, the mechanism of inflammation in CRS has still not been fully elucidated. The aim of the study was to investigate the role of interleukin-32 (IL-32), a recently discovered proinflammatory cytokine, in CRS. METHODS: We collected nasal epithelial cells and nasal tissue from patients with CRS and control subjects. We assayed mRNA for IL-32 by real-time PCR and measured IL-32 protein using ELISA, Western blot, and immunohistochemistry. RESULTS: The expression of mRNA for IL-32 was elevated in epithelial cells from uncinate tissue from patients with CRSsNP compared with patients with CRSwNP (P < 0.05), control subjects (P=0.06), and epithelial cells from nasal polyp (NP) tissue (P < 0.05). Production of IL-32 was induced by IFN-γ, TNF, and dsRNA in primary airway epithelial cells. In whole-tissue extracts, the expression of IL-32 protein was significantly elevated in patients with CRSwNP compared with patients with CRSsNP and control subjects. Immunohistochemistry data showed that IL-32 was detected in mucosal epithelial cells and inflammatory cells in the lamina propria. Levels of IL-32 were correlated with the levels of CD3 and macrophage mannose receptor in NP tissue. Immunofluorescence data showed IL-32 co-localization with CD3-positive T cells and CD68-positive macrophages in NPs. CONCLUSION: Overproduction of IL-32 may be involved in the pathogenesis of CRS, although the role of IL-32 in the inflammation in CRSsNP and CRSwNP may be different.
Authors: David D Tieu; Anju T Peters; Roderick G Carter; Roderick T Carter; Lydia Suh; David B Conley; Rakesh Chandra; James Norton; Leslie C Grammer; Kathleen E Harris; Atsushi Kato; Robert C Kern; Robert P Schleimer Journal: J Allergy Clin Immunol Date: 2010-03 Impact factor: 10.793
Authors: Claudia A Nold-Petry; Marcel F Nold; Jarod A Zepp; Soo-Hyun Kim; Norbert F Voelkel; Charles A Dinarello Journal: Proc Natl Acad Sci U S A Date: 2009-02-19 Impact factor: 11.205
Authors: C Bachert; N Van Bruaene; E Toskala; N Zhang; H Olze; G Scadding; C M Van Drunen; J Mullol; L Cardell; P Gevaert; T Van Zele; S Claeys; C Halldén; K Kostamo; U Foerster; M Kowalski; K Bieniek; A Olszewska-Ziaber; E Nizankowska-Mogilnicka; A Szczeklik; M Swierczynska; M Arcimowicz; V Lund; W Fokkens; T Zuberbier; C Akdis; G Canonica; P Van Cauwenberge; P Burney; J Bousquet Journal: Allergy Date: 2009-04 Impact factor: 13.146
Authors: Nicholas Van Bruaene; Lara Derycke; Claudina Angela Perez-Novo; Philippe Gevaert; Gabriele Holtappels; Natalie De Ruyck; Claude Cuvelier; Paul Van Cauwenberge; Claus Bachert Journal: J Allergy Clin Immunol Date: 2009-06-04 Impact factor: 10.793
Authors: Marcel F Nold; Claudia A Nold-Petry; Gregory B Pott; Jarod A Zepp; Milene T Saavedra; Soo-Hyun Kim; Charles A Dinarello Journal: J Immunol Date: 2008-07-01 Impact factor: 5.426
Authors: Chunwei Li; Li Shi; Yan Yan; Bruce R Gordon; William M Gordon; De-Yun Wang Journal: Curr Allergy Asthma Rep Date: 2013-04 Impact factor: 4.806
Authors: Cezmi A Akdis; Claus Bachert; Cemal Cingi; Mark S Dykewicz; Peter W Hellings; Robert M Naclerio; Robert P Schleimer; Dennis Ledford Journal: J Allergy Clin Immunol Date: 2013-04-12 Impact factor: 10.793
Authors: Jivianne T Lee; Oswaldo H Escobar; Rabin Anouseyan; Agnieszka Janisiewicz; Edward Eivers; Keith E Blackwell; David B Keschner; Rohit Garg; Edith Porter Journal: Int Forum Allergy Rhinol Date: 2014-09-07 Impact factor: 3.858
Authors: Karen Head; Lee Yee Chong; Claire Hopkins; Carl Philpott; Anne G M Schilder; Martin J Burton Journal: Cochrane Database Syst Rev Date: 2016-04-26
Authors: Lee Yee Chong; Karen Head; Claire Hopkins; Carl Philpott; Martin J Burton; Anne G M Schilder Journal: Cochrane Database Syst Rev Date: 2016-04-26