Literature DB >> 2189725

The mechanism of group I self-splicing: an internal guide sequence can be provided in trans.

A J Winter1, M J Groot Koerkamp, H F Tabak.   

Abstract

We have reconstituted a group I self-splicing reaction between two RNA molecules with different functional RNA parts: a substrate molecule containing the 5' splice site and a functional internal guide sequence (IGS), and a ribozyme molecule with core structure elements and splice sites but a mutated IGS. The 5' exon of the substrate molecule is ligated in trans to the 3' exon of the ribozyme molecule, suggesting that the deficient IGS in the ribozyme can be replaced by an externally added IGS present on the substrate molecule. This result is different from catalysis mediated by proteins where it is not possible to dissect the specificity of an enzyme from its catalytic activity.

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Year:  1990        PMID： 2189725      PMCID： PMC551900          DOI： 10.1002/j.1460-2075.1990.tb08319.x

Source DB:  PubMed          Journal:  EMBO J        ISSN： 0261-4189            Impact factor:   11.598


  20 in total

1.  Oligomerization of intervening sequence RNA molecules in the absence of proteins.

Authors:  A J Zaug; T R Cech
Journal:  Science       Date:  1985-09-13       Impact factor: 47.728

2.  Interlocked RNA circle formation by a self-splicing yeast mitochondrial group I intron.

Authors:  H F Tabak; G Van der Horst; A M Kamps; A C Arnberg
Journal:  Cell       Date:  1987-01-16       Impact factor: 41.582

Review 3.  Biological catalysis by RNA.

Authors:  T R Cech; B L Bass
Journal:  Annu Rev Biochem       Date:  1986       Impact factor: 23.643

4.  Enzymatic activity of the conserved core of a group I self-splicing intron.

Authors:  J W Szostak
Journal:  Nature       Date:  1986 Jul 3-9       Impact factor: 49.962

5.  Defining the inside and outside of a catalytic RNA molecule.

Authors:  J A Latham; T R Cech
Journal:  Science       Date:  1989-07-21       Impact factor: 47.728

6.  Catalysis of splicing-related reactions between dinucleotides by a ribozyme.

Authors:  P S Kay; T Inoue
Journal:  Nature       Date:  1987 May 28-Jun 3       Impact factor: 49.962

7.  Intermolecular exon ligation of the rRNA precursor of Tetrahymena: oligonucleotides can function as 5' exons.

Authors:  T Inoue; F X Sullivan; T R Cech
Journal:  Cell       Date:  1985-12       Impact factor: 41.582

8.  Assessment of a model for intron RNA secondary structure relevant to RNA self-splicing--a review.

Authors:  R B Waring; R W Davies
Journal:  Gene       Date:  1984-06       Impact factor: 3.688

9.  Making ends meet: a model for RNA splicing in fungal mitochondria.

Authors:  R W Davies; R B Waring; J A Ray; T A Brown; C Scazzocchio
Journal:  Nature       Date:  1982-12-23       Impact factor: 49.962

10.  Sites of circularization of the Tetrahymena rRNA IVS are determined by sequence and influenced by position and secondary structure.

Authors:  M D Been; T R Cech
Journal:  Nucleic Acids Res       Date:  1985-12-09       Impact factor: 16.971
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  3 in total

1.  A region of group I introns that contains universally conserved residues but is not essential for self-splicing.

Authors:  K P Williams; D N Fujimoto; T Inoue
Journal:  Proc Natl Acad Sci U S A       Date:  1992-11-01       Impact factor: 11.205

2.  Two group I ribozymes with different functions in a nuclear rDNA intron.

Authors:  W A Decatur; C Einvik; S Johansen; V M Vogt
Journal:  EMBO J       Date:  1995-09-15       Impact factor: 11.598

3.  Postsplicing-Derived Full-Length Intron Circles in the Protozoan Parasite Entamoeba histolytica.

Authors:  María S Mendoza-Figueroa; Eddy E Alfonso-Maqueira; Cristina Vélez; Elisa I Azuara-Liceaga; Selene Zárate; Nicolás Villegas-Sepúlveda; Odila Saucedo-Cárdenas; Jesús Valdés
Journal:  Front Cell Infect Microbiol       Date:  2018-08-03       Impact factor: 5.293
  3 in total

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