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Literature DB >> 218962

Single strand DNA binding of simian virus 40 tumor antigen.

Abstract

Simian virus 40 T antigen binds to both single and double strand DNA. The single and double strand DNA binding activity of crude T antigen preparations was evaluated by chromatography of the antigen on DNA-cellulose columns. Crude T antigen was retained on both native and denatured DNA-cellulos columns and was eluted from both columns under similar conditions. The interaction of highly purified T antigen with single and double strand DNA was evaluated by competition experiments using a DNA filter binding assay. These experiments showed that T antigen binds preferentially to single strand calf thymus DNA by more than an order of magnitude when compared to double strand calf thymus DNA.

Entities: Disease Species

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Year: 1979 PMID： 218962

Source DB: PubMed Journal: J Biol Chem ISSN： 0021-9258 Impact factor: 5.157

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Cited

20 in total

1. Nonspecific DNA binding activity of simian virus 40 large T antigen: evidence for the cooperation of two regions for full activity.

Authors: H J Lin; R H Upson; D T Simmons
Journal: J Virol Date: 1992-09 Impact factor: 5.103

2. Nonspecific double-stranded DNA binding activity of simian virus 40 large T antigen is involved in melting and unwinding of the origin.

Authors: Junfang Jiao; Daniel T Simmons
Journal: J Virol Date: 2003-12 Impact factor: 5.103

3. Simian virus 40 (SV40) T antigen binds specifically to double-stranded DNA but not to single-stranded DNA or DNA/RNA hybrids containing the SV40 regulatory sequences.

Authors: K J Auborn; R B Markowitz; E Wang; Y T Yu; C Prives
Journal: J Virol Date: 1988-06 Impact factor: 5.103

4. Analyses of the interaction between the origin binding domain from simian virus 40 T antigen and single-stranded DNA provide insights into DNA unwinding and initiation of DNA replication.

Authors: Danielle K Reese; Gretchen Meinke; Anuradha Kumar; Stephanie Moine; Kathleen Chen; James L Sudmeier; William Bachovchin; Andrew Bohm; Peter A Bullock
Journal: J Virol Date: 2006-09-27 Impact factor: 5.103

10. Topography of simian virus 40 A protein-DNA complexes: arrangement of pentanucleotide interaction sites at the origin of replication.

Authors: A L DeLucia; B A Lewton; R Tjian; P Tegtmeyer
Journal: J Virol Date: 1983-04 Impact factor: 5.103

Single strand DNA binding of simian virus 40 tumor antigen.

1. Nonspecific DNA binding activity of simian virus 40 large T antigen: evidence for the cooperation of two regions for full activity.

2. Nonspecific double-stranded DNA binding activity of simian virus 40 large T antigen is involved in melting and unwinding of the origin.

3. Simian virus 40 (SV40) T antigen binds specifically to double-stranded DNA but not to single-stranded DNA or DNA/RNA hybrids containing the SV40 regulatory sequences.

4. Analyses of the interaction between the origin binding domain from simian virus 40 T antigen and single-stranded DNA provide insights into DNA unwinding and initiation of DNA replication.

5. A lifetime of playing with enzymes.

6. Properties of the DNA-binding domain of the simian virus 40 large T antigen.

7. DNA binding properties of simian virus 40 temperature-sensitive A proteins.

8. Large T-antigen mutants define multiple steps in the initiation of simian virus 40 DNA replication.

9. Effects of T antigen and replication protein A on the initiation of DNA synthesis by DNA polymerase alpha-primase.

10. Topography of simian virus 40 A protein-DNA complexes: arrangement of pentanucleotide interaction sites at the origin of replication.