| Literature DB >> 21894897 |
Hong Y Cho1, Abiraman Srinivasan, Joanna Hong, Eric Hsu, Shiguang Liu, Arun Shrivats, Dan Kwak, Andrew K Bohaty, Hyun-Jong Paik, Jeffrey O Hollinger, Krzysztof Matyjaszewski.
Abstract
Star polymers with poly(ethylene glycol) (PEG) arms and a degradable cationic core were synthesized by the atom transfer radical copolymerization (ATRP) of poly(ethylene glycol) methyl ether methacrylate macromonomer (PEGMA), 2-(dimethylamino)ethyl methacrylate (DMAEMA), and a disulfide dimethacrylate (cross-linker, SS) via an "arm-first" approach. The star polymers had a diameter ~15 nm and were degraded under redox conditions by glutathione treatment into individual polymeric chains due to cleavage of the disulfide cross-linker, as confirmed by dynamic light scattering. The star polymers were cultured with mouse calvarial preosteoblast-like cells, embryonic day 1, subclone 4 (MC3T3-E1.4) to determine biocompatibility. Data suggest star polymers were biocompatible, with ≥ 80% cell viability after 48 h of incubation even at high concentration (800 μg/mL). Zeta potential values varied with N/P ratio confirming complexation with siRNA. Successful cellular uptake of the star polymers in MC3T3-E1.4 cells was observed by confocal microscopy and flow cytometry after 24 h of incubation.Entities:
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Year: 2011 PMID： 21894897 DOI： 10.1021/bm2006455
Source DB: PubMed Journal: Biomacromolecules ISSN： 1525-7797 Impact factor: 6.988