Literature DB >> 218911

ACTH radioimmunocytochemistry (RICH) on rat anterior pituitary cells.

G Rappay, M Kárteszi, G B Makara.   

Abstract

Radioimmunocytochemistry (RICH) was applied to detect corticotrophs in adult rat pituitaries and 8-day-old anterior pituitary monolayers by incubating sections and cultures with 125I-ACTH-anti ACTH immune complexes. After incubations autoradiography was made. In comparison, "conventional" immunostaining was carried out on adjacent sections and parallel cultures. It has been established that RICH is suitable for detection of corticotrophs.

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Year:  1979        PMID: 218911     DOI: 10.1007/bf00495668

Source DB:  PubMed          Journal:  Histochemistry        ISSN: 0301-5564


  7 in total

1.  Specific radioactivity of radioimmunoassay tracer determined by self-displacement: a re-evaluation.

Authors:  B J Morris
Journal:  Clin Chim Acta       Date:  1976-11-15       Impact factor: 3.786

2.  Radioimmunocytochemistry-a novel immunocytochemical principle.

Authors:  L I Larsson; T W Schwartz
Journal:  J Histochem Cytochem       Date:  1977-10       Impact factor: 2.479

3.  Growth and fine structure of monolayers derived from adult rat adenohypophyseal cell suspensions.

Authors:  G Rappay; A Gyévai; L Kondics; E Stark
Journal:  In Vitro       Date:  1973 Jan-Feb

4.  Classifications of anterior pituitary cell types with immunoenzyme histochemistry.

Authors:  P K Nakane
Journal:  J Histochem Cytochem       Date:  1970-01       Impact factor: 2.479

5.  Fixation of ejaculated spermatozoa for electron microscopy.

Authors:  M Stefanini; C De Martino; L Zamboni
Journal:  Nature       Date:  1967-10-14       Impact factor: 49.962

6.  Autoradiography and immunohistoperoxidase techniques applied to the same tissue section.

Authors:  N van Rooijen; J G Streefkerk
Journal:  J Immunol Methods       Date:  1976       Impact factor: 2.303

  7 in total
  1 in total

1.  Ultrastructural morphometry of the rat pituitary intermediate zone after stimulation and suppression.

Authors:  Wolfgang Saeger; Karl-Detlef John; Jörg Caselitz
Journal:  Endocr Pathol       Date:  1991-12       Impact factor: 3.943

  1 in total

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