UNLABELLED: Identifying sentinel nodes near the primary tumor remains a problem in, for example, head and neck cancer because of the limited resolution of current lymphoscintigraphic imaging when using (99m)Tc-nanocolloidal albumin. This study describes the development and evaluation of a nanocolloidal albumin-based tracer specifically dedicated for high-resolution PET detection. METHODS: (89)Zr was coupled to nanocolloidal albumin via the bifunctional chelate p-isothiocyanatobenzyldesferrioxamine B. Quality control tests, including particle size measurements, and in vivo biodistribution and imaging experiments in a rabbit lymphogenic metastasis model were performed. RESULTS: Coupling of (89)Zr to nanocolloidal albumin appeared to be efficient, resulting in a stable product with a radiochemical purity greater than 95%, without affecting the particle size. PET showed distinguished uptake of (89)Zr-nanocolloidal albumin in the sentinel nodes, with visualization of lymphatic vessels, and with a biodistribution comparable to (99m)Tc-nanocolloidal albumin. CONCLUSION: (89)Zr-nanocolloidal albumin is a promising tracer for sentinel node detection by PET.
UNLABELLED: Identifying sentinel nodes near the primary tumor remains a problem in, for example, head and neck cancer because of the limited resolution of current lymphoscintigraphic imaging when using (99m)Tc-nanocolloidal albumin. This study describes the development and evaluation of a nanocolloidal albumin-based tracer specifically dedicated for high-resolution PET detection. METHODS: (89)Zr was coupled to nanocolloidal albumin via the bifunctional chelate p-isothiocyanatobenzyldesferrioxamine B. Quality control tests, including particle size measurements, and in vivo biodistribution and imaging experiments in a rabbit lymphogenic metastasis model were performed. RESULTS: Coupling of (89)Zr to nanocolloidal albumin appeared to be efficient, resulting in a stable product with a radiochemical purity greater than 95%, without affecting the particle size. PET showed distinguished uptake of (89)Zr-nanocolloidal albumin in the sentinel nodes, with visualization of lymphatic vessels, and with a biodistribution comparable to (99m)Tc-nanocolloidal albumin. CONCLUSION: (89)Zr-nanocolloidal albumin is a promising tracer for sentinel node detection by PET.
Authors: Daniel L J Thorek; Diane S Abou; Bradley J Beattie; Rachel M Bartlett; Ruimin Huang; Pat B Zanzonico; Jan Grimm Journal: J Nucl Med Date: 2012-08-07 Impact factor: 10.057
Authors: Christina Bluemel; Domenico Rubello; Patrick M Colletti; Remco de Bree; Ken Herrmann Journal: Eur J Nucl Med Mol Imaging Date: 2015-04-28 Impact factor: 9.236
Authors: Daniel L J Thorek; David Ulmert; Ndeye-Fatou M Diop; Mihaela E Lupu; Michael G Doran; Ruimin Huang; Diane S Abou; Steven M Larson; Jan Grimm Journal: Nat Commun Date: 2014 Impact factor: 14.919
Authors: Derrek A Heuveling; Gerard W M Visser; Mattijs de Groot; Johannes F de Boer; Marian Baclayon; Wouter H Roos; Gijs J L Wuite; C René Leemans; Remco de Bree; Guus A M S van Dongen Journal: Eur J Nucl Med Mol Imaging Date: 2012-02-17 Impact factor: 9.236
Authors: Derrek A Heuveling; K Hakki Karagozoglu; Arthur Van Lingen; Otto S Hoekstra; Guus A M S Van Dongen; Remco De Bree Journal: EJNMMI Res Date: 2018-02-14 Impact factor: 3.138