Literature DB >> 2188993

Oligodeoxynucleoside phosphorothioate stability in subcellular extracts, culture media, sera and cerebrospinal fluid.

J M Campbell1, T A Bacon, E Wickstrom.   

Abstract

Degradation of a synthetic oligodeoxynucleoside phosphorothioate was studied in six systems used for antisense inhibition experiments. Oligodeoxynucleoside phosphorothioates were degraded very slowly at 37 degrees C in all of the systems studied. Measured half-lives of pentadecamers were 12 +/- 1 h in rabbit reticulocyte lysate, 7 +/- 1 h in HeLa cell postmitochondrial extract, 14 +/- 2 h in RPMI 1640 with 10% fetal bovine serum, 8 +/- 1 h in undiluted fetal bovine serum, 9 +/- 1 h in adult human serum, and 19 +/- 7 h in rat cerebrospinal fluid.

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Year:  1990        PMID： 2188993     DOI： 10.1016/0165-022x(90)90084-p

Source DB:  PubMed          Journal:  J Biochem Biophys Methods        ISSN： 0165-022X


  48 in total

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Review 5.  Gene modulation for treating liver fibrosis.

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7.  Newly designed six-membered azasugar nucleotide-containing phosphorothioate oligonucleotides as potent human immunodeficiency virus type 1 inhibitors.

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Authors:  K Oguro; N Oguro; T Kojima; S Y Grooms; A Calderone; X Zheng; M V Bennett; R S Zukin
Journal:  J Neurosci       Date:  1999-11-01       Impact factor: 6.167

9.  Stability and functional effectiveness of phosphorothioate modified duplex DNA and synthetic 'mini-genes'.

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Review 10.  DNA and RNA derivatives to optimize distribution and delivery.

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