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Literature DB >> 21889511

An improved cell isolation method for flow cytometric and functional analyses of cutaneous wound leukocytes.

Aleah L Brubaker¹, David F Schneider, Jessica L Palmer, Douglas E Faunce, Elizabeth J Kovacs.

Abstract

Isolation of leukocytes from full-thickness excisional wounds has proven to be a difficult process that results in poor cell yield and holds significant limitations for functional assays. Given the increased interest in the isolation, characterization and functional measurements of wound-derived cell populations, herein we describe a method for preparing wound cell suspensions with an improved yield that enables both phenotypic and functional assessments.

Entities: Chemical Disease Gene Species

Mesh：

Year: 2011 PMID： 21889511 PMCID： PMC3195830 DOI： 10.1016/j.jim.2011.08.013

Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303

11 in total

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Journal: J Investig Dermatol Symp Proc Date: 2006-09

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Authors: C Sepulveda-Merrill; S Mayall; A S Hamblin; S M Breathnach
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6. Prevention of NKT cell activation accelerates cutaneous wound closure and alters local inflammatory signals.

Authors: David F Schneider; Jessica L Palmer; Julia M Tulley; Elizabeth J Kovacs; Richard L Gamelli; Douglas E Faunce
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7. Modulation of macrophage phenotype by soluble product(s) released from neutrophils.

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Journal: J Immunol Date: 2005-02-15 Impact factor: 5.422

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Authors: Ankush Gosain; Richard L Gamelli; Luisa A DiPietro
Journal: J Surg Res Date: 2008-06-02 Impact factor: 2.192

9. A novel role for NKT cells in cutaneous wound repair.

Authors: David F Schneider; Jessica L Palmer; Julia M Tulley; John T Speicher; Elizabeth J Kovacs; Richard L Gamelli; Douglas E Faunce
Journal: J Surg Res Date: 2009-10-09 Impact factor: 2.192

10. The phenotype of murine wound macrophages.

Authors: Jean M Daley; Samielle K Brancato; Alan A Thomay; Jonathan S Reichner; Jorge E Albina
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4. Syndesome Therapeutics for Enhancing Diabetic Wound Healing.

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5. Mice engrafted with human hematopoietic stem cells support a human myeloid cell inflammatory response in vivo.

Authors: Andrew Baird; Chenliang Deng; Matthew H Eliceiri; Fatima Haghi; Xitong Dang; Raul Coimbra; Todd W Costantini; Bruce E Torbett; Brian P Eliceiri
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6. Reduced neutrophil chemotaxis and infiltration contributes to delayed resolution of cutaneous wound infection with advanced age.

Authors: Aleah L Brubaker; Juan L Rendon; Luis Ramirez; Mashkoor A Choudhry; Elizabeth J Kovacs
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