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Literature DB >> 21889501

Overexpression of UDP-GlcNAc transporter partially corrects galactosylation defect caused by UDP-Gal transporter mutation.

Dorota Maszczak-Seneczko¹, Teresa Olczak, Piotr Jakimowicz, Mariusz Olczak.

Abstract

Nucleotide sugar transporters deliver substrates for glycosyltransferases into the endoplasmic reticulum and the Golgi apparatus. We demonstrated that overexpression of UDP-GlcNAc transporter (NGT) in MDCK-RCA(r) and CHO-Lec8 mutant cells defective in UDP-Gal transporter (UGT) restored galactosylation of N-glycans. NGT overexpression resulted in decreased transport of UDP-GlcNAc into the Golgi vesicles. This effect resembled the phenotype of mutant cells corrected by UGT1 overexpression. The transport of UDP-Gal was not significantly changed. Our data suggest that the biological function of UGT and NGT in galactosylation of macromolecules may be coupled.

Entities: Chemical Disease Gene

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Year: 2011 PMID： 21889501 DOI： 10.1016/j.febslet.2011.08.038

Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124

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Cited

11 in total

1. Galactose supplementation enhance sialylation of recombinant Fc-fusion protein in CHO cell: an insight into the role of galactosylation in sialylation.

Authors: Jintao Liu; Jie Wang; Li Fan; Xinning Chen; Dongdong Hu; Xiancun Deng; H Fai Poon; Haibin Wang; Xuping Liu; Wen-Song Tan
Journal: World J Microbiol Biotechnol Date: 2015-05-01 Impact factor: 3.312

2. SLC35A2-CDG: Functional characterization, expanded molecular, clinical, and biochemical phenotypes of 30 unreported Individuals.

Authors: Bobby G Ng; Paulina Sosicka; Satish Agadi; Mohammed Almannai; Carlos A Bacino; Rita Barone; Lorenzo D Botto; Jennifer E Burton; Colleen Carlston; Brian Hon-Yin Chung; Julie S Cohen; David Coman; Katrina M Dipple; Naghmeh Dorrani; William B Dobyns; Abdallah F Elias; Leon Epstein; William A Gahl; Domenico Garozzo; Trine Bjørg Hammer; Jaclyn Haven; Delphine Héron; Matthew Herzog; George E Hoganson; Jesse M Hunter; Mahim Jain; Jane Juusola; Shenela Lakhani; Hane Lee; Joy Lee; Katherine Lewis; Nicola Longo; Charles Marques Lourenço; Christopher C Y Mak; Dianalee McKnight; Bryce A Mendelsohn; Cyril Mignot; Ghayda Mirzaa; Wendy Mitchell; Hiltrud Muhle; Stanley F Nelson; Mariusz Olczak; Christina G S Palmer; Arthur Partikian; Marc C Patterson; Tyler M Pierson; Shane C Quinonez; Brigid M Regan; M Elizabeth Ross; Maria J Guillen Sacoto; Fernando Scaglia; Ingrid E Scheffer; Devorah Segal; Nilika Shah Singhal; Pasquale Striano; Luisa Sturiale; Joseph D Symonds; Sha Tang; Eric Vilain; Mary Willis; Lynne A Wolfe; Hui Yang; Shoji Yano; Zöe Powis; Sharon F Suchy; Jill A Rosenfeld; Andrew C Edmondson; Stephanie Grunewald; Hudson H Freeze
Journal: Hum Mutat Date: 2019-04-24 Impact factor: 4.878

3. UDP-galactose (SLC35A2) and UDP-N-acetylglucosamine (SLC35A3) Transporters Form Glycosylation-related Complexes with Mannoside Acetylglucosaminyltransferases (Mgats).

Authors: Dorota Maszczak-Seneczko; Paulina Sosicka; Beata Kaczmarek; Michał Majkowski; Marcin Luzarowski; Teresa Olczak; Mariusz Olczak
Journal: J Biol Chem Date: 2015-05-05 Impact factor: 5.157

4. Biosynthesis of GlcNAc-rich N- and O-glycans in the Golgi apparatus does not require the nucleotide sugar transporter SLC35A3.

Authors: Bozena Szulc; Paulina Sosicka; Dorota Maszczak-Seneczko; Edyta Skurska; Auhen Shauchuk; Teresa Olczak; Hudson H Freeze; Mariusz Olczak
Journal: J Biol Chem Date: 2020-09-16 Impact factor: 5.157

5. UDP-N-acetylglucosamine transporter (SLC35A3) regulates biosynthesis of highly branched N-glycans and keratan sulfate.

Authors: Dorota Maszczak-Seneczko; Paulina Sosicka; Teresa Olczak; Piotr Jakimowicz; Michał Majkowski; Mariusz Olczak
Journal: J Biol Chem Date: 2013-06-13 Impact factor: 5.157

Overexpression of UDP-GlcNAc transporter partially corrects galactosylation defect caused by UDP-Gal transporter mutation.

1. Galactose supplementation enhance sialylation of recombinant Fc-fusion protein in CHO cell: an insight into the role of galactosylation in sialylation.

2. SLC35A2-CDG: Functional characterization, expanded molecular, clinical, and biochemical phenotypes of 30 unreported Individuals.

3. UDP-galactose (SLC35A2) and UDP-N-acetylglucosamine (SLC35A3) Transporters Form Glycosylation-related Complexes with Mannoside Acetylglucosaminyltransferases (Mgats).

4. Biosynthesis of GlcNAc-rich N- and O-glycans in the Golgi apparatus does not require the nucleotide sugar transporter SLC35A3.

5. UDP-N-acetylglucosamine transporter (SLC35A3) regulates biosynthesis of highly branched N-glycans and keratan sulfate.

6. Modified secreted alkaline phosphatase as an improved reporter protein for N-glycosylation analysis.

7. SLC35A2-CDG: Novel variant and review.

Review 8. Determinants of Glycosaminoglycan (GAG) Structure.

Review 9. Structure and function of nucleotide sugar transporters: Current progress.

10. Lysine at position 329 within a C-terminal dilysine motif is crucial for the ER localization of human SLC35B4.