Literature DB >> 21889496

Regioselectivity of human UDP-glucuronosyltransferase isozymes in flavonoid biotransformation by metal complexation and tandem mass spectrometry.

Scott A Robotham1, Jennifer S Brodbelt.   

Abstract

Based on reactions with five flavonoids, the regioselectivities of twelve human UDP-glucuronosyltransferase (UGT) isozymes were elucidated. The various flavonoid glucuronides were differentiated based on LC-MS/MS fragmentation patterns of [Co(II)(flavonoid-H)(4,7-diphenyl-1,10-phenanthroline)(2)](+) complexes generated upon post-column complexation. Glucuronide distributions were evaluated to allow a systematic assessment of the regioselectivity of each isozyme. The various UGT enzymes, including eight UGT1A and four UGT2B, displayed a remarkable range of selectivities, both in terms of the positions of glucuronidation and relative reactivity with flavanones versus flavonols. Copyright Â
© 2011 Elsevier Inc. All rights reserved.

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Year:  2011        PMID： 21889496      PMCID： PMC3205324          DOI： 10.1016/j.bcp.2011.08.015

Source DB:  PubMed          Journal:  Biochem Pharmacol        ISSN： 0006-2952            Impact factor:   5.858


  35 in total

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Review 6.  Bioavailability and bioefficacy of polyphenols in humans. I. Review of 97 bioavailability studies.

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7.  Glucuronidation of flavonoids by recombinant UGT1A3 and UGT1A9.

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Journal:  Biochem Pharmacol       Date:  2008-05-15       Impact factor: 5.858

8.  Identification and characterization of polyphenolic antioxidants using on-line liquid chromatography, electrochemistry, and electrospray ionization tandem mass spectrometry.

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9.  Application of preparative high-speed counter-current chromatography/electrospray ionization mass spectrometry for a fast screening and fractionation of polyphenols.

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10.  Gas-phase hydrogen/deuterium exchange and conformations of deprotonated flavonoids and gas-phase acidities of flavonoids.

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  3 in total

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Journal:  J Nat Prod       Date:  2013-05-29       Impact factor: 4.050

2.  Identification of flavone glucuronide isomers by metal complexation and tandem mass spectrometry: regioselectivity of uridine 5'-diphosphate-glucuronosyltransferase isozymes in the biotransformation of flavones.

Authors:  Scott A Robotham; Jennifer S Brodbelt
Journal:  J Agric Food Chem       Date:  2013-02-08       Impact factor: 5.279

3.  Mutual regioselective inhibition of human UGT1A1-mediated glucuronidation of four flavonoids.

Authors:  Guo Ma; Baojian Wu; Song Gao; Zhen Yang; Yong Ma; Ming Hu
Journal:  Mol Pharm       Date:  2013-07-17       Impact factor: 4.939
  3 in total

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