AIMS: Increasing evidence suggests an association between oestrogens and colorectal cancer. Oestrogen receptor beta, ER-β, putatively plays a pathobiological role in colorectal cancer as colorectal epithelial cells frequently express ER-β. The aim was to elucidate the association of the dinucleotide (CA) repeat polymorphism of the ER-β gene (ESR2) with colorectal cancer. METHODS AND RESULTS: Deoxyribonucleic acids extracted from the renal cortex of 1488 Japanese autopsies with complete clinical/pathological data were studied. CA repeat polymorphism was determined by polymerase chain reaction using fluorescein-labelled primers. Patients were divided into three genotype groups according to the number of CA repeats of each allele (S < 22, L ≥ 22); SS (with two S alleles), SL (with one each S and L allele) and LL (with two L alleles). The presence/absence of colorectal cancers was determined by examining the clinical records and autopsy material. The incidence of colorectal cancer was significantly different according to the ESR2 CA repeat genotype only among females (SS, 37/202 = 18.3%; SL, 19/332 = 5.7%; LL, 5/155 = 3.2%, P < 0.0001). Immunohistochemically, cancers in females with the SS genotype, but not the SL genotype, frequently expressed the C-terminus portion of ER-β1 (wild-type ER-β). CONCLUSIONS: A role for ESR2 CA repeat polymorphism in the pathogenesis of colorectal cancer among females is suggested.
AIMS: Increasing evidence suggests an association between oestrogens and colorectal cancer. Oestrogen receptor beta, ER-β, putatively plays a pathobiological role in colorectal cancer as colorectal epithelial cells frequently express ER-β. The aim was to elucidate the association of the dinucleotide (CA) repeat polymorphism of the ER-β gene (ESR2) with colorectal cancer. METHODS AND RESULTS: Deoxyribonucleic acids extracted from the renal cortex of 1488 Japanese autopsies with complete clinical/pathological data were studied. CA repeat polymorphism was determined by polymerase chain reaction using fluorescein-labelled primers. Patients were divided into three genotype groups according to the number of CA repeats of each allele (S < 22, L ≥ 22); SS (with two S alleles), SL (with one each S and L allele) and LL (with two L alleles). The presence/absence of colorectal cancers was determined by examining the clinical records and autopsy material. The incidence of colorectal cancer was significantly different according to the ESR2 CA repeat genotype only among females (SS, 37/202 = 18.3%; SL, 19/332 = 5.7%; LL, 5/155 = 3.2%, P < 0.0001). Immunohistochemically, cancers in females with the SS genotype, but not the SL genotype, frequently expressed the C-terminus portion of ER-β1 (wild-type ER-β). CONCLUSIONS: A role for ESR2 CA repeat polymorphism in the pathogenesis of colorectal cancer among females is suggested.
Authors: A Joan Levine; Ugonna Ihenacho; Won Lee; Jane C Figueiredo; David J Vandenberg; Christopher K Edlund; Brian D Davis; Mariana C Stern; Robert W Haile Journal: Int J Colorectal Dis Date: 2012-05-30 Impact factor: 2.571
Authors: Michael N Passarelli; Amanda I Phipps; John D Potter; Karen W Makar; Anna E Coghill; Karen J Wernli; Emily White; Andrew T Chan; Carolyn M Hutter; Ulrike Peters; Polly A Newcomb Journal: Cancer Res Date: 2012-11-13 Impact factor: 12.701
Authors: Anja Rudolph; Hong Shi; Asta Försti; Michael Hoffmeister; Juan Sainz; Lina Jansen; Kari Hemminki; Hermann Brenner; Jenny Chang-Claude Journal: BMC Cancer Date: 2014-11-07 Impact factor: 4.430