AIMS: In 2005, a Francisella sp. was isolated from diseased cultured giant abalone (Haliotis gigantea) in Japan. The aim of this study was to clarify the taxonomic status of this Francisella sp. Shimane-1 isolate in relation to the four described Francisella species. METHODS AND RESULTS: The 16S rRNA gene and several housekeeping genes of the Shimane-1 were compared to isolates of the four recognized species within the Francisella genus. DNA-DNA hybridization (DDH) and biochemical profile comparison were performed with the two phylogenetically closely related species, Francisella philomiragia and Francisella noatunensis. Results show that the Shimane-1 is genetically different from all described Francisella species and differs phenotypically from F. philomiragia and F. noatunensis. The average DDH similarity of Francisella sp. Shimane-1 to F. noatunensis ssp. noatunensis (NCIMB14265(T)) and to F. philomiragia (DSM7535(T)) was 49·2 and 61%, respectably, clearly supporting the establishment of Shimane-1 as a new species within the Francisella genus. CONCLUSIONS: The phenotypic and genetic results presented in this study suggest the establishment of Shimane-1 as a novel species, for which the name Francisella halioticida sp. nov. (=LMG26062(T), =DSM23729(T)) is proposed. SIGNIFICANCE AND IMPACT OF THE STUDY: This study clarifies the taxonomic position and characteristics of a novel mollusc pathogenic Francisella species.
AIMS: In 2005, a Francisella sp. was isolated from diseased cultured giant abalone (Haliotis gigantea) in Japan. The aim of this study was to clarify the taxonomic status of this Francisella sp. Shimane-1 isolate in relation to the four described Francisella species. METHODS AND RESULTS: The 16S rRNA gene and several housekeeping genes of the Shimane-1 were compared to isolates of the four recognized species within the Francisella genus. DNA-DNA hybridization (DDH) and biochemical profile comparison were performed with the two phylogenetically closely related species, Francisella philomiragia and Francisella noatunensis. Results show that the Shimane-1 is genetically different from all described Francisella species and differs phenotypically from F. philomiragia and F. noatunensis. The average DDH similarity of Francisella sp. Shimane-1 to F. noatunensis ssp. noatunensis (NCIMB14265(T)) and to F. philomiragia (DSM7535(T)) was 49·2 and 61%, respectably, clearly supporting the establishment of Shimane-1 as a new species within the Francisella genus. CONCLUSIONS: The phenotypic and genetic results presented in this study suggest the establishment of Shimane-1 as a novel species, for which the name Francisella halioticida sp. nov. (=LMG26062(T), =DSM23729(T)) is proposed. SIGNIFICANCE AND IMPACT OF THE STUDY: This study clarifies the taxonomic position and characteristics of a novel mollusc pathogenic Francisella species.
Authors: Jean F Challacombe; Jeannine M Petersen; La Verne Gallegos-Graves; David Hodge; Segaran Pillai; Cheryl R Kuske Journal: Appl Environ Microbiol Date: 2017-01-17 Impact factor: 4.792
Authors: Esteban Soto; Matt J Griffin; Juan Alberto Morales; Elías Barquero Calvo; Fernanda de Alexandre Sebastião; Adrián Lopez Porras; Xindy Víquez-Rodríguez; Stephen R Reichley; Thomas G Rosser; Cynthia Ware; Barbara A Byrne; Julio C García; Benjamin R LaFrentz; Alvin C Camus Journal: Appl Environ Microbiol Date: 2018-08-01 Impact factor: 4.792
Authors: Jordy P M Coolen; Andreas Sjödin; Boulos Maraha; Gerard F Hajer; Mats Forsman; Ellen Verspui; Hendrina M E Frenay; Daan W Notermans; Maaike C de Vries; Frans A G Reubsaet; Armand Paauw; Guus Roeselers Journal: Stand Genomic Sci Date: 2013-08-10
Authors: José G Ramírez-Paredes; Kim D Thompson; Matthijs Metselaar; Khalid Shahin; Esteban Soto; Randolph H Richards; David J Penman; Duncan J Colquhoun; Alexandra Adams Journal: Front Microbiol Date: 2017-12-12 Impact factor: 5.640