Taro Matsushita1, Yoshinori Kusakabe, Akihiro Kitamura, Sakie Okada, Kenya Murase. 1. Department of Medical Physics and Engineering, Division of Medical Technology and Science, Faculty of Health Science, Graduate School of Medicine, Osaka University, 1-7 Yamadaoka, Suita, Osaka 565-0871, Japan.
Abstract
PURPOSE: The aim of this study was to assess the mechanism of the protective effect of hydrogen-rich water (HW) against cisplatin (CP)-induced nephrotoxicity in rats using blood oxygenation level-dependent (BOLD) magnetic resonance imaging (MRI). MATERIALS AND METHODS: Apparent transverse relaxation time-weighted images (T2 WI) were acquired in 28 rats. The control group (n = 7) had free access to standard water (SW) and no CP injection. The CP group (n = 7) had free access to SW and was given a CP injection on day 0. The CP+HW group (n = 7) had free access to HW and had a CP injection. The HW group (n = 7) had free access to HW and no CP injection. The apparent transverse relaxation rate (R2) was estimated from T2 WI. RESULTS: In the CP+HW group, the R2 value in the medulla normalized by the value of the day 0 was significantly greater than that in the CP group on days 4 and 7. The creatinine and blood urea nitrogen levels in the CP group were significantly higher than those in the control, CP+HW, and HW groups. CONCLUSION: BOLD MRI may be useful for demonstrating the change in R2 in CP-induced nephrotoxicity in rats. The changes in the CP+HW group were suspected to be due to a reduction of cytotoxic oxygen radicals.
PURPOSE: The aim of this study was to assess the mechanism of the protective effect of hydrogen-rich water (HW) against cisplatin (CP)-induced nephrotoxicity in rats using blood oxygenation level-dependent (BOLD) magnetic resonance imaging (MRI). MATERIALS AND METHODS: Apparent transverse relaxation time-weighted images (T2 WI) were acquired in 28 rats. The control group (n = 7) had free access to standard water (SW) and no CP injection. The CP group (n = 7) had free access to SW and was given a CP injection on day 0. The CP+HW group (n = 7) had free access to HW and had a CP injection. The HW group (n = 7) had free access to HW and no CP injection. The apparent transverse relaxation rate (R2) was estimated from T2 WI. RESULTS: In the CP+HW group, the R2 value in the medulla normalized by the value of the day 0 was significantly greater than that in the CP group on days 4 and 7. The creatinine and blood ureanitrogen levels in the CP group were significantly higher than those in the control, CP+HW, and HW groups. CONCLUSION: BOLD MRI may be useful for demonstrating the change in R2 in CP-induced nephrotoxicity in rats. The changes in the CP+HW group were suspected to be due to a reduction of cytotoxic oxygen radicals.
Authors: Anette E Fransson; Marta Kisiel; Kristian Pirttilä; Curt Pettersson; Pernilla Videhult Pierre; Göran F E Laurell Journal: Front Cell Neurosci Date: 2017-09-13 Impact factor: 5.505
Authors: Ahmed E Zayed; Ahmed Saleh; Asmaa M S Gomaa; Mahmoud Abd-Elkareem; Mamdouh M Anwar; Khaled M A Hassanein; Mohsen M Elsherbiny; Ahmed M Kotb Journal: Oxid Med Cell Longev Date: 2018-06-11 Impact factor: 6.543