| Literature DB >> 21880816 |
Jennifer Andris-Widhopf, Peter Steinberger, Roberta Fuller, Christoph Rader, Carlos F Barbas.
Abstract
The development of therapeutic antibodies for use in the treatment of human diseases has long been a goal for many researchers in the antibody field. One way to obtain these antibodies is through phage-display libraries constructed from human lymphocytes. This protocol describes the construction of human scFv (single chain antibody fragment) libraries using a short linker (GGSSRSS) or a long linker (GGSSRSSSSGGGGSGGGG). In this method, the individual rearranged heavy- and light-chain variable regions are amplified separately and are linked through a series of overlap polymerase chain reaction (PCR) steps to give the final scFv products that are used for cloning.Entities:
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Year: 2011 PMID： 21880816 DOI： 10.1101/pdb.prot065573
Source DB: PubMed Journal: Cold Spring Harb Protoc ISSN： 1559-6095