Literature DB >> 21880811

Single-virus tracking in live cells.

Michael J Rust, Melike Lakadamyali, Boerries Brandenburg, Xiaowei Zhuang.   

Abstract

Real-time, live-cell imaging techniques and single-particle tracking algorithms can be used to follow individual virus particles as they infect cells. This article describes the labeling of viruses and cellular structures with fluorescent probes to allow visualization in live cells. It also discusses how virus trajectories and virus-cell interactions can be imaged and analyzed. Methods used for time-lapse imaging are outlined, as are inhibitors and reagents used to study the role of the cellular machinery during viral infection. Algorithms for tracking virus particles and obtaining quantitative measurements of viral transport and virus-cell interactions are also included.

Entities:  

Mesh：

Year:  2011        PMID： 21880811     DOI： 10.1101/pdb.top065623

Source DB:  PubMed          Journal:  Cold Spring Harb Protoc        ISSN： 1559-6095


  8 in total

1.  Dissecting the Cell Entry Pathway of Baculovirus by Single-Particle Tracking and Quantitative Electron Microscopic Analysis.

Authors:  Fujun Qin; Congrui Xu; Jia Hu; Chengfeng Lei; Zhenhua Zheng; Ke Peng; Hanzhong Wang; Xiulian Sun
Journal:  J Virol       Date:  2019-04-03       Impact factor: 5.103

Review 2.  Particle tracking in drug and gene delivery research: State-of-the-art applications and methods.

Authors:  Benjamin S Schuster; Laura M Ensign; Daniel B Allan; Jung Soo Suk; Justin Hanes
Journal:  Adv Drug Deliv Rev       Date:  2015-04-07       Impact factor: 15.470

Review 3.  Molecular determinants of the ratio of inert to infectious virus particles.

Authors:  P J Klasse
Journal:  Prog Mol Biol Transl Sci       Date:  2014-12-01       Impact factor: 3.622

4.  Single-Particle Detection of Transcription following Rotavirus Entry.

Authors:  Eric N Salgado; Srigokul Upadhyayula; Stephen C Harrison
Journal:  J Virol       Date:  2017-08-24       Impact factor: 5.103

5.  Dynamics of transmissible gastroenteritis virus internalization unraveled by single-virus tracking in live cells.

Authors:  Jian Wang; Yangyang Li; Shouyu Wang; Fei Liu
Journal:  FASEB J       Date:  2020-02-04       Impact factor: 5.191

6.  Real-Time Visualization and Quantification of Human Cytomegalovirus Replication in Living Cells Using the ANCHOR DNA Labeling Technology.

Authors:  Bernard Mariamé; Sandrine Kappler-Gratias; Martin Kappler; Stéphanie Balor; Franck Gallardo; Kerstin Bystricky
Journal:  J Virol       Date:  2018-08-29       Impact factor: 5.103

7.  Porcine rotavirus mainly infects primary porcine enterocytes at the basolateral surface.

Authors:  Tingting Cui; Sebastiaan Theuns; Jiexiong Xie; Hans J Nauwynck
Journal:  Vet Res       Date:  2019-12-19       Impact factor: 3.683

8.  Phototracking Vaccinia Virus Transport Reveals Dynamics of Cytoplasmic Dispersal and a Requirement for A36R and F12L for Exit from the Site of Wrapping.

Authors:  Helena Lynn; Liam M Howell; Russell J Diefenbach; Timothy P Newsome
Journal:  Viruses       Date:  2018-07-24       Impact factor: 5.048
  8 in total

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