| Literature DB >> 2187748 |
Abstract
A novel enzyme for DNA dephosphorylation, HK phosphatase, is completely and irreversibly inactivated at 65 degrees C. HK phosphatase treatment of BamHI-digested pBR322 reduces nonrecombinants in cloning experiments to approx. 5% of transformant colonies.Mesh：
Substances：
Year: 1990 PMID： 2187748 DOI： 10.1016/0378-1119(90)90064-x
Source DB: PubMed Journal: Gene ISSN： 0378-1119 Impact factor: 3.688