Literature DB >> 2186929

A novel and sensitive assay for phospholipase D in intact cells.

R W Randall1, R W Bonser, N T Thompson, L G Garland.   

Abstract

A novel and sensitive assay for phospholipase D (PLD) that measures the incorporation of high specific activity [3H]butan-1-ol into [3H]phosphatidylbutanol has been developed. The assay has been used to measure PLD activation in human neutrophils and platelets. Both the chemotactic peptide fMet-Leu-Phe and opsonised-zymosan stimulated PLD in the human neutrophil. In the platelet, PLD was stimulated by thrombin and collagen but responses were small and only occurred at high agonist concentrations. This assay has a number of advantages over existing techniques and should be valuable for investigating PLD activation in a variety of isolated cells and possibly intact tissues.

Entities:  

Mesh：

Substances：

Year:  1990        PMID： 2186929     DOI： 10.1016/0014-5793(90)80772-b

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  17 in total

Review 1.  Chemical modulation of glycerolipid signaling and metabolic pathways.

Authors:  Sarah A Scott; Thomas P Mathews; Pavlina T Ivanova; Craig W Lindsley; H Alex Brown
Journal:  Biochim Biophys Acta       Date:  2014-01-15

2.  Evidence that v-Src-induced phospholipase D activity is mediated by a G protein.

Authors:  H Jiang; K Alexandropoulos; J Song; D A Foster
Journal:  Mol Cell Biol       Date:  1994-06       Impact factor: 4.272

3.  Vasopressin-stimulated [3H]-inositol phosphate and [3H]-phosphatidylbutanol accumulation in A10 vascular smooth muscle cells.

Authors:  R Plevin; A Stewart; A Paul; M J Wakelam
Journal:  Br J Pharmacol       Date:  1992-09       Impact factor: 8.739

4.  Opioid peptides activate phospholipase D and protein kinase C-epsilon in chicken embryo neuron cultures.

Authors:  D Mangoura; G Dawson
Journal:  Proc Natl Acad Sci U S A       Date:  1993-04-01       Impact factor: 11.205

5.  v-Src increases diacylglycerol levels via a type D phospholipase-mediated hydrolysis of phosphatidylcholine.

Authors:  J G Song; L M Pfeffer; D A Foster
Journal:  Mol Cell Biol       Date:  1991-10       Impact factor: 4.272

6.  TLC and 31P-NMR analysis of low polarity phospholipids.

Authors:  Mikhail Vyssotski; Andrew MacKenzie; Dawn Scott
Journal:  Lipids       Date:  2008-12-13       Impact factor: 1.880

7.  Tyrosine phosphorylation is involved in receptor coupling to phospholipase D but not phospholipase C in the human neutrophil.

Authors:  I J Uings; N T Thompson; R W Randall; G D Spacey; R W Bonser; A T Hudson; L G Garland
Journal:  Biochem J       Date:  1992-02-01       Impact factor: 3.857

8.  Early signalling events implicated in leukotriene B4-induced activation of the NADPH oxidase in eosinophils: role of Ca2+, protein kinase C and phospholipases C and D.

Authors:  R S Perkins; M A Lindsay; P J Barnes; M A Giembycz
Journal:  Biochem J       Date:  1995-09-15       Impact factor: 3.857

9.  Thrombin induces a biphasic 1,2-diacylglycerol production in human platelets.

Authors:  S Nakashima; A Suganuma; A Matsui; Y Nozawa
Journal:  Biochem J       Date:  1991-04-15       Impact factor: 3.857

10.  Demethoxyviridin and wortmannin block phospholipase C and D activation in the human neutrophil.

Authors:  R W Bonser; N T Thompson; R W Randall; J E Tateson; G D Spacey; H F Hodson; L G Garland
Journal:  Br J Pharmacol       Date:  1991-05       Impact factor: 8.739
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.