Literature DB >> 21865427

Quantification of Clostridium difficile in antibiotic-associated-diarrhea patients.

Paul Naaber1, Jelena Stsepetova, Imbi Smidt, Merle Rätsep, Siiri Kõljalg, Krista Lõivukene, Liis Jaanimäe, Iren H Löhr, Olav B Natås, Kai Truusalu, Epp Sepp.   

Abstract

Comparing culture- and non-culture-based methods for quantifying Clostridium difficile in antibiotic-associated-diarrhea patients, we found that the real-time PCR method correlated well with quantitative culture and was more sensitive. A positive association between the population levels of C. difficile and the presence of its toxins was found.

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Year:  2011        PMID： 21865427      PMCID： PMC3187345          DOI： 10.1128/JCM.05115-11

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  17 in total

Review 1.  Treatment of Clostridium difficile-associated disease: old therapies and new strategies.

Authors:  Saima Aslam; Richard J Hamill; Daniel M Musher
Journal:  Lancet Infect Dis       Date:  2005-09       Impact factor: 25.071

2.  Measurement of fecal lactoferrin as a marker of fecal leukocytes.

Authors:  R L Guerrant; V Araujo; E Soares; K Kotloff; A A Lima; W H Cooper; A G Lee
Journal:  J Clin Microbiol       Date:  1992-05       Impact factor: 5.948

3.  Quantification of Bifidobacterium spp., Escherichia coli and Clostridium difficile in faecal samples of breast-fed and formula-fed infants by real-time PCR.

Authors:  John Penders; Cornelis Vink; Christel Driessen; Nancy London; Carel Thijs; Ellen E Stobberingh
Journal:  FEMS Microbiol Lett       Date:  2005-02-01       Impact factor: 2.742

4.  Fecal lactoferrin, interleukin-1beta, and interleukin-8 are elevated in patients with severe Clostridium difficile colitis.

Authors:  T S Steiner; C A Flores; T T Pizarro; R L Guerrant
Journal:  Clin Diagn Lab Immunol       Date:  1997-11

5.  Characterization of human intestinal bifidobacteria using competitive PCR and PCR-TTGE.

Authors:  Irène Mangin; Antonia Suau; Fabien Magne; Daniel Garrido; Martin Gotteland; Christel Neut; Philippe Pochart
Journal:  FEMS Microbiol Ecol       Date:  2006-01       Impact factor: 4.194

6.  Correlation of disease severity with fecal toxin levels in patients with Clostridium difficile-associated diarrhea and distribution of PCR ribotypes and toxin yields in vitro of corresponding isolates.

Authors:  Thomas Akerlund; Bo Svenungsson; Asa Lagergren; Lars G Burman
Journal:  J Clin Microbiol       Date:  2006-02       Impact factor: 5.948

7.  Novel evidence suggesting Clostridium difficile is present in human gut microbiota more frequently than previously suspected.

Authors:  Masahiro Iizuka; Shiho Konno; Hiroaki Itou; Junichi Chihara; Itaru Toyoshima; Yasuo Horie; Kenji Sasaki; Akiko Sato; Kenichi Shindo; Sumio Watanabe
Journal:  Microbiol Immunol       Date:  2004       Impact factor: 1.955

Review 8.  European Society of Clinical Microbiology and Infectious Diseases (ESCMID): data review and recommendations for diagnosing Clostridium difficile-infection (CDI).

Authors:  M J T Crobach; O M Dekkers; M H Wilcox; E J Kuijper
Journal:  Clin Microbiol Infect       Date:  2009-12       Impact factor: 8.067

9.  Concurrence of Clostridium difficile toxin A enzyme-linked immunosorbent assay, fecal lactoferrin assay, and clinical criteria with C. difficile cytotoxin titer in two patient cohorts.

Authors:  M A Schleupner; D C Garner; K M Sosnowski; C J Schleupner; L J Barrett; E Silva; D Hirsch; R L Guerrant
Journal:  J Clin Microbiol       Date:  1995-07       Impact factor: 5.948

10.  Development of an extensive set of 16S rDNA-targeted primers for quantification of pathogenic and indigenous bacteria in faecal samples by real-time PCR.

Authors:  T Rinttilä; A Kassinen; E Malinen; L Krogius; A Palva
Journal:  J Appl Microbiol       Date:  2004       Impact factor: 3.772
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  16 in total

1.  Prevalence and duration of asymptomatic Clostridium difficile carriage among healthy subjects in Pittsburgh, Pennsylvania.

Authors:  Alison L Galdys; Jemma S Nelson; Kathleen A Shutt; Jessica L Schlackman; Diana L Pakstis; A William Pasculle; Jane W Marsh; Lee H Harrison; Scott R Curry
Journal:  J Clin Microbiol       Date:  2014-04-23       Impact factor: 5.948

2.  Role of fecal Clostridium difficile load in discrepancies between toxin tests and PCR: is quantitation the next step in C. difficile testing?

Authors:  J L Leslie; S H Cohen; J V Solnick; C R Polage
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2012-07-20       Impact factor: 3.267

3.  Potential of real-time PCR threshold cycle (CT) to predict presence of free toxin and clinically relevant C. difficile infection (CDI) in patients with cancer.

Authors:  Mini Kamboj; Jennifer Brite; Tracy McMillen; Elizabeth Robilotti; Alejandro Herrera; Kent Sepkowitz; N Esther Babady
Journal:  J Infect       Date:  2017-12-08       Impact factor: 6.072

4.  Dual Reporting of Clostridioides difficile PCR and Predicted Toxin Result Based on PCR Cycle Threshold Reduces Treatment of Toxin-Negative Patients without Increases in Adverse Outcomes.

Authors:  Matthew M Hitchcock; Marisa Holubar; Catherine A Hogan; Lucy S Tompkins; Niaz Banaei
Journal:  J Clin Microbiol       Date:  2019-10-23       Impact factor: 5.948

5.  Laboratory diagnosis of Clostridium difficile infection: Comparison of Techlab C. diff Quik Chek Complete, Xpert C. difficile, and multistep algorithmic approach.

Authors:  Ja Young Seo; Ji Hun Jeong; Kyung Hee Kim; Jeong-Yeal Ahn; Pil-Whan Park; Yiel-Hea Seo
Journal:  J Clin Lab Anal       Date:  2017-02-08       Impact factor: 2.352

6.  Longitudinal Investigation of Carriage Rates, Counts, and Genotypes of Toxigenic Clostridium difficile in Early Infancy.

Authors:  Hiroyuki Kubota; Hiroshi Makino; Agata Gawad; Akira Kushiro; Eiji Ishikawa; Takafumi Sakai; Takuya Akiyama; Kazunori Matsuda; Rocio Martin; Jan Knol; Kenji Oishi
Journal:  Appl Environ Microbiol       Date:  2016-09-16       Impact factor: 4.792

7.  Correlation between Clostridium difficile bacterial load, commercial real-time PCR cycle thresholds, and results of diagnostic tests based on enzyme immunoassay and cell culture cytotoxicity assay.

Authors:  Léa-Laurence Dionne; Frédéric Raymond; Jacques Corbeil; Jean Longtin; Philippe Gervais; Yves Longtin
Journal:  J Clin Microbiol       Date:  2013-08-21       Impact factor: 5.948

8.  Development of TaqMan-based quantitative PCR for sensitive and selective detection of toxigenic Clostridium difficile in human stools.

Authors:  Hiroyuki Kubota; Takafumi Sakai; Agata Gawad; Hiroshi Makino; Takuya Akiyama; Eiji Ishikawa; Kenji Oishi
Journal:  PLoS One       Date:  2014-10-31       Impact factor: 3.240

9.  Comparison of Diagnostic Algorithms for Detecting Toxigenic Clostridium difficile in Routine Practice at a Tertiary Referral Hospital in Korea.

Authors:  Hee-Won Moon; Hyeong Nyeon Kim; Mina Hur; Hee Sook Shim; Heejung Kim; Yeo-Min Yun
Journal:  PLoS One       Date:  2016-08-17       Impact factor: 3.240

10.  Early Clostridium difficile infection during allogeneic hematopoietic stem cell transplantation.

Authors:  Melissa A Kinnebrew; Yeon Joo Lee; Robert R Jenq; Lauren Lipuma; Eric R Littmann; Asia Gobourne; Daniel No; Marcel van den Brink; Eric G Pamer; Ying Taur
Journal:  PLoS One       Date:  2014-03-24       Impact factor: 3.240
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