CONTEXT: Activin A, myostatin, and follistatin have recently emerged as important regulatory molecules of reproduction and the musculoskeletal system. Little is known, however, about their day/night patterns of secretion and their physiological regulation by energy availability. OBJECTIVE: The objective of the study was to explore day/night patterns of secretion and assess whether energy deprivation alters circulating levels of activin A, myostatin, follistatin, and cortisol and to examine whether leptin may mediate this effect. DESIGN, SETTING AND PATIENTS, AND INTERVENTIONS:Seven healthy lean men (aged 23.2 ± 3.7 yr, body mass index 23.6 ± 1.7 kg/m(2)) were studied for 72 h under three different conditions: on their baseline/isocaloric diet and in a complete fasting state with administration of either placebo or metreleptin. The two fasting studies were randomized and double blinded. Blood samples were obtained every 15 min from 0800 h on d 3 until 0800 h on d 4 and pooled hourly. MAIN OUTCOME MEASURES: Serum concentrations of activin A, myostatin, follistatin, cortisol, and leptin were measured. RESULTS: In contrast to cortisol, we demonstrated no day/night pattern of activin A, myostatin, and follistatin secretion. Activin A concentrations decreased significantly in response to energy deprivation (P < 0.01). Follistatin and cortisol concentrations increased significantly (P < 0.01 and P < 0.01, respectively). Myostatin remained unaffected (P = 0.40). Leptin administration reversed cortisol response (P < 0.01) but failed to alter activin A, follistatin, or myostatin concentrations. CONCLUSIONS: Unlike cortisol, there is no day/night variation in the concentrations of activin A, myostatin, and follistatin in healthy young males. Although energy deprivation-induced cortisol changes are leptin mediated, the changes in follistatin and activin A concentrations occur through a leptin-independent pathway.
RCT Entities:
CONTEXT: Activin A, myostatin, and follistatin have recently emerged as important regulatory molecules of reproduction and the musculoskeletal system. Little is known, however, about their day/night patterns of secretion and their physiological regulation by energy availability. OBJECTIVE: The objective of the study was to explore day/night patterns of secretion and assess whether energy deprivation alters circulating levels of activin A, myostatin, follistatin, and cortisol and to examine whether leptin may mediate this effect. DESIGN, SETTING AND PATIENTS, AND INTERVENTIONS: Seven healthy lean men (aged 23.2 ± 3.7 yr, body mass index 23.6 ± 1.7 kg/m(2)) were studied for 72 h under three different conditions: on their baseline/isocaloric diet and in a complete fasting state with administration of either placebo or metreleptin. The two fasting studies were randomized and double blinded. Blood samples were obtained every 15 min from 0800 h on d 3 until 0800 h on d 4 and pooled hourly. MAIN OUTCOME MEASURES: Serum concentrations of activin A, myostatin, follistatin, cortisol, and leptin were measured. RESULTS: In contrast to cortisol, we demonstrated no day/night pattern of activin A, myostatin, and follistatin secretion. Activin A concentrations decreased significantly in response to energy deprivation (P < 0.01). Follistatin and cortisol concentrations increased significantly (P < 0.01 and P < 0.01, respectively). Myostatin remained unaffected (P = 0.40). Leptin administration reversed cortisol response (P < 0.01) but failed to alter activin A, follistatin, or myostatin concentrations. CONCLUSIONS: Unlike cortisol, there is no day/night variation in the concentrations of activin A, myostatin, and follistatin in healthy young males. Although energy deprivation-induced cortisol changes are leptin mediated, the changes in follistatin and activin A concentrations occur through a leptin-independent pathway.
Authors: Jean L Chan; Joseph E Mietus; Patricia M Raciti; Ary L Goldberger; Christos S Mantzoros Journal: Clin Endocrinol (Oxf) Date: 2007-01 Impact factor: 3.478
Authors: Elizabeth Sienkiewicz; Faidon Magkos; Konstantinos N Aronis; Mary Brinkoetter; John P Chamberland; Sharon Chou; Kalliopi M Arampatzi; Chuanyun Gao; Anastasia Koniaris; Christos S Mantzoros Journal: Metabolism Date: 2011-07-07 Impact factor: 8.694
Authors: Sharon H Chou; John P Chamberland; Xiaowen Liu; Giuseppe Matarese; Chuanyun Gao; Rianna Stefanakis; Mary T Brinkoetter; Huizhi Gong; Kalliopi Arampatzi; Christos S Mantzoros Journal: Proc Natl Acad Sci U S A Date: 2011-04-04 Impact factor: 11.205
Authors: Jennifer N Cash; Carlis A Rejon; Alexandra C McPherron; Daniel J Bernard; Thomas B Thompson Journal: EMBO J Date: 2009-07-30 Impact factor: 11.598
Authors: I Sadaf Farooqi; Giuseppe Matarese; Graham M Lord; Julia M Keogh; Elizabeth Lawrence; Chizo Agwu; Veronica Sanna; Susan A Jebb; Francesco Perna; Silvia Fontana; Robert I Lechler; Alex M DePaoli; Stephen O'Rahilly Journal: J Clin Invest Date: 2002-10 Impact factor: 14.808
Authors: Angela K Odle; Helen Beneš; Andrea Melgar Castillo; Noor Akhter; Mohsin Syed; Anessa Haney; Melody Allensworth-James; Linda Hardy; Benjamin Winter; Ragul Manoharan; Raiyan Syed; Melanie C MacNicol; Angus M MacNicol; Gwen V Childs Journal: Endocrinology Date: 2018-02-01 Impact factor: 4.736
Authors: John P Chamberland; Reena L Berman; Konstantinos N Aronis; Christos S Mantzoros Journal: Eur J Endocrinol Date: 2013-09-13 Impact factor: 6.664
Authors: Joo Young Huh; Grigorios Panagiotou; Vassilis Mougios; Mary Brinkoetter; Maria T Vamvini; Benjamin E Schneider; Christos S Mantzoros Journal: Metabolism Date: 2012-09-25 Impact factor: 8.694