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Literature DB >> 2186371

PCR-induced (ligase-free) subcloning: a rapid reliable method to subclone polymerase chain reaction (PCR) products.

A R Shuldiner¹, L A Scott, J Roth.

Abstract

Mesh：

Substances：
Oligonucleotide Probes

Year: 1990 PMID： 2186371 PMCID： PMC330643 DOI： 10.1093/nar/18.7.1920

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

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2 in total

1. A general method of in vitro preparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions.

Authors: R Higuchi; B Krummel; R K Saiki
Journal: Nucleic Acids Res Date: 1988-08-11 Impact factor: 16.971

2. Direct cloning and sequence analysis of enzymatically amplified genomic sequences.

Authors: S J Scharf; G T Horn; H A Erlich
Journal: Science Date: 1986-09-05 Impact factor: 47.728

2 in total

15 in total

1. Ligation-independent cloning of PCR products (LIC-PCR).

Authors: C Aslanidis; P J de Jong
Journal: Nucleic Acids Res Date: 1990-10-25 Impact factor: 16.971

2. Improved cloning efficiency of polymerase chain reaction (PCR) products after proteinase K digestion.

Authors: J S Crowe; H J Cooper; M A Smith; M J Sims; D Parker; D Gewert
Journal: Nucleic Acids Res Date: 1991-01-11 Impact factor: 16.971

3. Construction of T-vectors, a rapid and general system for direct cloning of unmodified PCR products.

Authors: D Marchuk; M Drumm; A Saulino; F S Collins
Journal: Nucleic Acids Res Date: 1991-03-11 Impact factor: 16.971

4. Competitive priming PCR: a versatile method to generate cohesive terminus.

Authors: Jin-Shui Pan; Zhi-Ping Zhang; Jia-Yan Cai; Jing Dong; Jian-Lin Ren; Xiao-Zhong Wang
Journal: Mol Biol Rep Date: 2009-03-31 Impact factor: 2.316

Review 5. Design of Experiments As a Tool for Optimization in Recombinant Protein Biotechnology: From Constructs to Crystals.

Authors: Christos Papaneophytou
Journal: Mol Biotechnol Date: 2019-12 Impact factor: 2.695

10. Construction of a Rhizopus arrhizus glucoamylase gene suitable for expression in distinct host: introns spliced artificially by PCR.

Authors: Liquan Yang; Xiaojun Dai; Jianhua Hou; Chunxiao Ma; Cuiyan Wang; Zhiqiang Wu; Minggang Li
Journal: Mol Biol Rep Date: 2007-06-20 Impact factor: 2.316

PCR-induced (ligase-free) subcloning: a rapid reliable method to subclone polymerase chain reaction (PCR) products.

1. A general method of in vitro preparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions.

2. Direct cloning and sequence analysis of enzymatically amplified genomic sequences.

1. Ligation-independent cloning of PCR products (LIC-PCR).

2. Improved cloning efficiency of polymerase chain reaction (PCR) products after proteinase K digestion.

3. Construction of T-vectors, a rapid and general system for direct cloning of unmodified PCR products.

4. Competitive priming PCR: a versatile method to generate cohesive terminus.

Review 5. Design of Experiments As a Tool for Optimization in Recombinant Protein Biotechnology: From Constructs to Crystals.

6. PCR-mediated recombination and mutagenesis. SOEing together tailor-made genes.

7. Construction of recombinant DNA by exonuclease recession.

8. DISEC-TRISEC: di- and trinucleotide-sticky-end cloning of PCR-amplified DNA.

9. Kinasing PCR products for efficient blunt-end cloning and linker addition.

10. Construction of a Rhizopus arrhizus glucoamylase gene suitable for expression in distinct host: introns spliced artificially by PCR.