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Literature DB >> 2186174

Spleen necrosis virus gag polyprotein is necessary for particle assembly and release but not for proteolytic processing.

T A Weaver¹, K J Talbot, A T Panganiban.

Abstract

The nature of spleen necrosis virus pol gene expression and the role of gag and gag-pol polyproteins in virion assembly was investigated. The DNA sequence of the gag-pol junction revealed that the two genes occupy the same open reading frame but are separated by an in-frame amber stop codon. Biochemical analysis of gag-pol translational readthrough in vitro and in Escherichia coli suggests that, in a manner similar to that in other mammalian type C retroviruses, amber stop codon suppression is required for pol gene expression. Removal of the gag stop codon had little or no effect on synthesis or cleavage of the polyprotein but interrupted particle assembly. This block could be overcome by complementation with wild-type gag protein.

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Year: 1990 PMID： 2186174 PMCID： PMC249442

Source DB: PubMed Journal: J Virol ISSN： 0022-538X Impact factor: 5.103

56 in total

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Journal: J Natl Cancer Inst Date: 1973-08 Impact factor: 13.506

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Journal: Proc Natl Acad Sci U S A Date: 1973-06 Impact factor: 11.205

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Journal: Virology Date: 1969-07 Impact factor: 3.616

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Journal: Nat New Biol Date: 1971-09-15

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Journal: Anal Biochem Date: 1980-11-15 Impact factor: 3.365

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Authors: J J Dunn; F W Studier
Journal: J Mol Biol Date: 1983-06-05 Impact factor: 5.469

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Journal: Nature Date: 1982-01-07 Impact factor: 49.962

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Journal: Proc Natl Acad Sci U S A Date: 1983-09 Impact factor: 11.205

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Journal: J Virol Date: 1984-03 Impact factor: 5.103

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Journal: Proc Natl Acad Sci U S A Date: 1988-11 Impact factor: 11.205

23 in total

Review 1. Misreading of termination codons in eukaryotes by natural nonsense suppressor tRNAs.

Authors: H Beier; M Grimm
Journal: Nucleic Acids Res Date: 2001-12-01 Impact factor: 16.971

2. Transposition of a Ty3 GAG3-POL3 fusion mutant is limited by availability of capsid protein.

Authors: J Kirchner; S B Sandmeyer; D B Forrest
Journal: J Virol Date: 1992-10 Impact factor: 5.103

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Authors: S Oertle; N Bowles; P F Spahr
Journal: J Virol Date: 1992-06 Impact factor: 5.103

4. Foamy virus Pol protein expressed as a Gag-Pol fusion retains enzymatic activities, allowing for infectious virus production.

Authors: Eun-Gyung Lee; Amber Sinicrope; Dana L Jackson; Shuyuarn F Yu; Maxine L Linial
Journal: J Virol Date: 2012-04-04 Impact factor: 5.103

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Authors:
Journal: Nucleic Acids Res Date: 1991-04-25 Impact factor: 16.971

6. Bipartite signal for read-through suppression in murine leukemia virus mRNA: an eight-nucleotide purine-rich sequence immediately downstream of the gag termination codon followed by an RNA pseudoknot.

Authors: Y X Feng; H Yuan; A Rein; J G Levin
Journal: J Virol Date: 1992-08 Impact factor: 5.103