H Lahlaoui1, S Dahmen, M B Moussa, B Omrane. 1. laboratory of microbiology, Department of biology, Military Hospital of Tunis, Tunis, Tunisia. hella_lahlaoui@yahoo.fr
Abstract
PURPOSE: To study the resistance to third-generation cephalosporins in Providencia stuartii strain isolated from hospitalized patient in Tunisia and to identify the responsible genes MATERIALS AND METHODS: This strain was analysed by PCR and sequencing to identify the genes responsible for the β-lactamase resistance phenotypes. The transferability of the phenotypes was tested by conjugation to Escherichia coli J53. The isoelectric point was determinate by isoelectrofocalisation. RESULTS: This resistance was carried by a 60 kb plasmid that encoded a β-lactamase with a pI of 5.4. This β-lactamase revealed identity with the blaTEM-1 gene encoding the TEM-1 β-lactamase, except for a replacement of the Val residue at position 84 by Ile, and the Ala residue at position 184 by Val. These two mutations were encountered in TEM-116 β-lactamase. CONCLUSION: This study demonstrates the first description of TEM-116 in the P. stuartii species in the world and the first one in a Tunisian hospital.
PURPOSE: To study the resistance to third-generation cephalosporins in Providencia stuartii strain isolated from hospitalized patient in Tunisia and to identify the responsible genes MATERIALS AND METHODS: This strain was analysed by PCR and sequencing to identify the genes responsible for the β-lactamase resistance phenotypes. The transferability of the phenotypes was tested by conjugation to Escherichia coli J53. The isoelectric point was determinate by isoelectrofocalisation. RESULTS: This resistance was carried by a 60 kb plasmid that encoded a β-lactamase with a pI of 5.4. This β-lactamase revealed identity with the blaTEM-1 gene encoding the TEM-1 β-lactamase, except for a replacement of the Val residue at position 84 by Ile, and the Ala residue at position 184 by Val. These two mutations were encountered in TEM-116 β-lactamase. CONCLUSION: This study demonstrates the first description of TEM-116 in the P. stuartii species in the world and the first one in a Tunisian hospital.
Authors: Marcelo Silva Folhas Damas; Roumayne Lopes Ferreira; Emeline Boni Campanini; Gabriela Guerrera Soares; Leslie Camelo Campos; Pedro Mendes Laprega; Andrea Soares da Costa; Caio César de Melo Freire; André Pitondo-Silva; Louise Teixeira Cerdeira; Anderson Ferreira da Cunha; Maria-Cristina da Silva Pranchevicius Journal: Front Med (Lausanne) Date: 2022-07-28
Authors: Manuel Ares-Arroyo; Cristina Bernabe-Balas; Alfonso Santos-Lopez; Maria R Baquero; Kashi N Prasad; Dolores Cid; Carmen Martin-Espada; Alvaro San Millan; Bruno Gonzalez-Zorn Journal: Front Microbiol Date: 2018-03-16 Impact factor: 5.640