Literature DB >> 21859541

Determination of some phenolic compounds in red wine by RP-HPLC: method development and validation.

Vívian Maria Burin1, Stefany Grützmann Arcari, Léa Luzia Freitas Costa, Marilde T Bordignon-Luiz.   

Abstract

A methodology employing reversed-phase high-performance liquid chromatography (RP-HPLC) was developed and validated for simultaneous determination of five phenolic compounds in red wine. The chromatographic separation was carried out in a C(18) column with water acidify with acetic acid (pH 2.6) (solvent A) and 20% solvent A and 80% acetonitrile (solvent B) as the mobile phase. The validation parameters included: selectivity, linearity, range, limits of detection and quantitation, precision and accuracy, using an internal standard. All calibration curves were linear (R(2) > 0.999) within the range, and good precision (RSD < 2.6%) and recovery (80-120%) was obtained for all compounds. This method was applied to quantify phenolics in red wine samples from Santa Catarina State, Brazil, and good separation peaks for phenolic compounds in these wines were observed.

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Year:  2011        PMID： 21859541     DOI： 10.1093/chrsci/49.8.647

Source DB:  PubMed          Journal:  J Chromatogr Sci        ISSN： 0021-9665            Impact factor:   1.618


  11 in total

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