Youhai Dong1, Xujun Chen, Minghai Wang, Yang Hong. 1. Department of Orthopaedics, The Fifth People's Hospital of Fudan University, Shanghai, People's Republic of China. youhaidong1964@163.com
Abstract
STUDY DESIGN: A rabbit laminectomy model was used to evaluate the efficacy of artificial laminae of vertebral arch using bone marrow-derived osteoblasts transplanted in a collagen sponge. OBJECTIVE: The objective of this study is to reconstruct the artificial laminae of vertebral arch using bone marrow-derived osteoblasts transplanted in a collagen sponge on a rabbit model. SUMMARY OF BACKGROUND DATA: Because the laminectomy and semilaminectomy can effectively decompress the spinal cord and expand the vertebral canal, they have been performed as routine surgical procedures. However, long-term follow-up results show that these procedures can lead to many serious complications. A variety of strategies have been used to solve these complications, but there are few experiments to determine the efficacy of reconstructing the laminae of vertebral arch using bone marrow-derived osteoblasts and the collagen sponge. METHODS: The bone marrow mesenchymal stem cells (BMSCs) from the bone marrow in the femur of 2-week-old rabbits were obtained by centrifugation and adhesion. The BMSCs were induced to differentiate into osteoblasts, which were transplanted into collagen sponge to construct the tissue-engineering bone. A total of 48 rabbits were randomly divided into three groups. Lumbar laminectomies were performed on all of the rabbits. Group A was the control. Groups B and C were implanted with collagen sponge and tissue-engineering bone, respectively. The artificial laminae of the vertebral arch were examined qualitatively by imageology and histomorphometry. RESULTS: The artificial laminae of the vertebral arch successfully formed 4 weeks after the operation in group C; computed tomography examination at 4 weeks showed that the new laminae of vertebral arch were formed, and that the vertebral canal was intact. CONCLUSION: The artificial laminae of the vertebral arch can be successfully constructed using tissue engineering of transplanted BMSCs.
STUDY DESIGN: A rabbit laminectomy model was used to evaluate the efficacy of artificial laminae of vertebral arch using bone marrow-derived osteoblasts transplanted in a collagen sponge. OBJECTIVE: The objective of this study is to reconstruct the artificial laminae of vertebral arch using bone marrow-derived osteoblasts transplanted in a collagen sponge on a rabbit model. SUMMARY OF BACKGROUND DATA: Because the laminectomy and semilaminectomy can effectively decompress the spinal cord and expand the vertebral canal, they have been performed as routine surgical procedures. However, long-term follow-up results show that these procedures can lead to many serious complications. A variety of strategies have been used to solve these complications, but there are few experiments to determine the efficacy of reconstructing the laminae of vertebral arch using bone marrow-derived osteoblasts and the collagen sponge. METHODS: The bone marrow mesenchymal stem cells (BMSCs) from the bone marrow in the femur of 2-week-old rabbits were obtained by centrifugation and adhesion. The BMSCs were induced to differentiate into osteoblasts, which were transplanted into collagen sponge to construct the tissue-engineering bone. A total of 48 rabbits were randomly divided into three groups. Lumbar laminectomies were performed on all of the rabbits. Group A was the control. Groups B and C were implanted with collagen sponge and tissue-engineering bone, respectively. The artificial laminae of the vertebral arch were examined qualitatively by imageology and histomorphometry. RESULTS: The artificial laminae of the vertebral arch successfully formed 4 weeks after the operation in group C; computed tomography examination at 4 weeks showed that the new laminae of vertebral arch were formed, and that the vertebral canal was intact. CONCLUSION: The artificial laminae of the vertebral arch can be successfully constructed using tissue engineering of transplanted BMSCs.
Authors: Nuno A Silva; Rui A Sousa; Joana S Fraga; Marco Fontes; Hugo Leite-Almeida; Rui Cerqueira; Armando Almeida; Nuno Sousa; Rui L Reis; Antonio J Salgado Journal: Tissue Eng Part C Methods Date: 2012-08-20 Impact factor: 3.056
Authors: Juan N Albiñana-Cunningham; Purificación Ripalda-Cemboráin; Tania Labiano; José I Echeveste; Froilán Granero-Moltó; Matías Alfonso-Olmos Journal: J Orthop Surg Res Date: 2018-04-05 Impact factor: 2.359