Literature DB >> 21856849

Vestibules are part of the substrate path in the multidrug efflux transporter AcrB of Escherichia coli.

Fasahath Husain1, Mihir Bikhchandani, Hiroshi Nikaido.   

Abstract

The path of substrates in the multidrug efflux pump AcrB of Escherichia coli was examined by using labeling with a lipophilic substrate mimic, Bodipy FL maleimide. Four (out of eight) residues in the vestibule bound the dye, suggesting its role in substrate transport, whereas only one (out of nine) residue in the central cavity tested positive.

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Year:  2011        PMID： 21856849      PMCID： PMC3187200          DOI： 10.1128/JB.05759-11

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  17 in total

1.  Interplay between efflux pumps may provide either additive or multiplicative effects on drug resistance.

Authors:  A Lee; W Mao; M S Warren; A Mistry; K Hoshino; R Okumura; H Ishida; O Lomovskaya
Journal:  J Bacteriol       Date:  2000-06       Impact factor: 3.490

2.  Structural basis of multiple drug-binding capacity of the AcrB multidrug efflux pump.

Authors:  Edward W Yu; Gerry McDermott; Helen I Zgurskaya; Hiroshi Nikaido; Daniel E Koshland
Journal:  Science       Date:  2003-05-09       Impact factor: 47.728

3.  Promiscuous partnering and independent activity of MexB, the multidrug transporter protein from Pseudomonas aeruginosa.

Authors:  Alexander Welch; Chidiebere U Awah; Shiheng Jing; Hendrik W van Veen; Henrietta Venter
Journal:  Biochem J       Date:  2010-09-01       Impact factor: 3.857

4.  Mutations in the central cavity and periplasmic domain affect efflux activity of the resistance-nodulation-division pump EmhB from Pseudomonas fluorescens cLP6a.

Authors:  Elizabeth M Hearn; Murray R Gray; Julia M Foght
Journal:  J Bacteriol       Date:  2006-01       Impact factor: 3.490

5.  Crystal structures of a multidrug transporter reveal a functionally rotating mechanism.

Authors:  Satoshi Murakami; Ryosuke Nakashima; Eiki Yamashita; Takashi Matsumoto; Akihito Yamaguchi
Journal:  Nature       Date:  2006-08-16       Impact factor: 49.962

6.  Structural asymmetry of AcrB trimer suggests a peristaltic pump mechanism.

Authors:  Markus A Seeger; André Schiefner; Thomas Eicher; François Verrey; Kay Diederichs; Klaas M Pos
Journal:  Science       Date:  2006-09-01       Impact factor: 47.728

Review 7.  Multidrug efflux pumps of gram-negative bacteria.

Authors:  H Nikaido
Journal:  J Bacteriol       Date:  1996-10       Impact factor: 3.490

8.  A periplasmic drug-binding site of the AcrB multidrug efflux pump: a crystallographic and site-directed mutagenesis study.

Authors:  Edward W Yu; Julio R Aires; Gerry McDermott; Hiroshi Nikaido
Journal:  J Bacteriol       Date:  2005-10       Impact factor: 3.490

9.  Aminoglycosides are captured from both periplasm and cytoplasm by the AcrD multidrug efflux transporter of Escherichia coli.

Authors:  Julio Ramos Aires; Hiroshi Nikaido
Journal:  J Bacteriol       Date:  2005-03       Impact factor: 3.490

10.  CAVER: a new tool to explore routes from protein clefts, pockets and cavities.

Authors:  Martin Petrek; Michal Otyepka; Pavel Banás; Pavlína Kosinová; Jaroslav Koca; Jirí Damborský
Journal:  BMC Bioinformatics       Date:  2006-06-22       Impact factor: 3.169
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  21 in total

1.  Transport of drugs by the multidrug transporter AcrB involves an access and a deep binding pocket that are separated by a switch-loop.

Authors:  Thomas Eicher; Hi-jea Cha; Markus A Seeger; Lorenz Brandstätter; Jasmin El-Delik; Jürgen A Bohnert; Winfried V Kern; François Verrey; Markus G Grütter; Kay Diederichs; Klaas M Pos
Journal:  Proc Natl Acad Sci U S A       Date:  2012-03-26       Impact factor: 11.205

2.  Reversal of the Drug Binding Pocket Defects of the AcrB Multidrug Efflux Pump Protein of Escherichia coli.

Authors:  Ketaki Soparkar; Alfred D Kinana; Jon W Weeks; Keith D Morrison; Hiroshi Nikaido; Rajeev Misra
Journal:  J Bacteriol       Date:  2015-08-03       Impact factor: 3.490

3.  Conserved small protein associates with the multidrug efflux pump AcrB and differentially affects antibiotic resistance.

Authors:  Errett C Hobbs; Xuefeng Yin; Brian J Paul; Jillian L Astarita; Gisela Storz
Journal:  Proc Natl Acad Sci U S A       Date:  2012-09-24       Impact factor: 11.205

4.  A fluorescent microplate assay quantifies bacterial efflux and demonstrates two distinct compound binding sites in AcrB.

Authors:  Ramkumar Iyer; Annette Ferrari; R Rijnbrand; Alice L Erwin
Journal:  Antimicrob Agents Chemother       Date:  2015-02-02       Impact factor: 5.191

Review 5.  The challenge of efflux-mediated antibiotic resistance in Gram-negative bacteria.

Authors:  Xian-Zhi Li; Patrick Plésiat; Hiroshi Nikaido
Journal:  Clin Microbiol Rev       Date:  2015-04       Impact factor: 26.132

6.  Molecular mechanism of MBX2319 inhibition of Escherichia coli AcrB multidrug efflux pump and comparison with other inhibitors.

Authors:  Attilio V Vargiu; Paolo Ruggerone; Timothy J Opperman; Son T Nguyen; Hiroshi Nikaido
Journal:  Antimicrob Agents Chemother       Date:  2014-08-11       Impact factor: 5.191

Review 7.  Strategic Moves of "Superbugs" Against Available Chemical Scaffolds: Signaling, Regulation, and Challenges.

Authors:  Bikash Baral; M R Mozafari
Journal:  ACS Pharmacol Transl Sci       Date:  2020-04-13

8.  AcrB drug-binding pocket substitution confers clinically relevant resistance and altered substrate specificity.

Authors:  Jessica M A Blair; Vassiliy N Bavro; Vito Ricci; Niraj Modi; Pierpaolo Cacciotto; Ulrich Kleinekathӧfer; Paolo Ruggerone; Attilio V Vargiu; Alison J Baylay; Helen E Smith; Yvonne Brandon; David Galloway; Laura J V Piddock
Journal:  Proc Natl Acad Sci U S A       Date:  2015-03-03       Impact factor: 11.205

9.  Crystal structure of AcrB complexed with linezolid at 3.5 Å resolution.

Authors:  Li-Wei Hung; Heung-Bok Kim; Satoshi Murakami; Goutam Gupta; Chang-Yub Kim; Thomas C Terwilliger
Journal:  J Struct Funct Genomics       Date:  2013-05-15

10.  Directed evolution of an E. coli inner membrane transporter for improved efflux of biofuel molecules.

Authors:  Jee Loon Foo; Susanna Su Jan Leong
Journal:  Biotechnol Biofuels       Date:  2013-05-21       Impact factor: 6.040
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