Mike Mackness1, Bharti Mackness. 1. PONTECH ART, Calle de L'Argelagar, 43840 Salou, Tarragona, Spain. mike.mackness@gmail.com
Abstract
OBJECTIVES: To investigate the effects of dilution on high-density lipoprotein (HDL) associated paraoxonase-1 (PON1) activity. DESIGN AND METHODS: HDL was prepared by ultracentrifugation, diluted with TRIS buffer pH 8.2 (0-128 fold) and assayed spectrophotometrically for PON1 activity using paraoxon and phenyl acetate. RESULTS: HDL-PON1 activity increased progressively with dilution to over 200% of the undiluted activity by 128 fold dilution (P<0.001). Conversely HDL size decreased. CONCLUSION: These results indicate the urgent need to standardise conditions for measuring PON1 activity.
OBJECTIVES: To investigate the effects of dilution on high-density lipoprotein (HDL) associated paraoxonase-1 (PON1) activity. DESIGN AND METHODS: HDL was prepared by ultracentrifugation, diluted with TRIS buffer pH 8.2 (0-128 fold) and assayed spectrophotometrically for PON1 activity using paraoxon and phenyl acetate. RESULTS: HDL-PON1 activity increased progressively with dilution to over 200% of the undiluted activity by 128 fold dilution (P<0.001). Conversely HDL size decreased. CONCLUSION: These results indicate the urgent need to standardise conditions for measuring PON1 activity.