| Literature DB >> 21852814 |
Åsa Anveden1, Kajsa Sjöholm, Peter Jacobson, Vilborg Palsdottir, Andrew J Walley, Philippe Froguel, Nasser Al-Daghri, Philip G McTernan, Niklas Mejhert, Peter Arner, Lars Sjöström, Lena M S Carlsson, Per-Arne Svensson.
Abstract
Adipocytes secrete many proteins that regulate metabolic functions. The gene inter-α (globulin) inhibitor H5 (ITIH-5) encodes a secreted protein and is known to be expressed abundantly in the placenta. However, using gene expression profiles data we observed high expression of ITIH-5 in adipose tissue. The aim of this study was to test the hypothesis that ITIH-5 is strongly expressed in human adipocytes and adipose tissue, and is related to obesity and clinical metabolic variables. ITIH-5 adipose tissue mRNA expression was analyzed with DNA microarray and real-time PCR, and its association with clinical variables was examined. ITIH-5 protein expression was analyzed using western blot. ITIH-5 mRNA expression was abundant in human adipose tissue, adipocytes, and placenta, and higher in subcutaneous (sc) compared to omental adipose tissue (P < 0.0001). ITIH-5 mRNA and protein expression in sc adipose tissue were higher in obese compared to lean subjects (P < 0.0001 and P < 0.001, respectively). ITIH-5 mRNA expression was reduced after diet-induced weight loss (P < 0.0001). ITIH-5 mRNA expression was associated with anthropometry and clinical metabolic variables. In conclusion, ITIH-5 is highly expressed in sc adipose tissue, increased in obesity, down regulated after weight loss, and associated with measures of body size and metabolism. Together, this indicates that ITIH-5 merits further investigation as a regulator of human metabolism.Entities:
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Year: 2011 PMID： 21852814 DOI： 10.1038/oby.2011.268
Source DB: PubMed Journal: Obesity (Silver Spring) ISSN： 1930-7381 Impact factor: 5.002