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Literature DB >> 21851837

Chlorimuron ethyl as a new selectable marker for disrupting genes in the insect-pathogenic fungus Metarhizium robertsii.

Liangcai Lin¹, Fengqing Wang, Dongzhi Wei.

Abstract

A lack of selectable markers was a hindrance in investigating gene function in Metarhizium robertsii. A reliable Agrobacterium-mediated transformation system based on the use of chlorimuron ethyl as the selectable marker was developed which could serve as a useful tool to inactivate genes involved in insect pathogenicity.

Entities: Chemical Species

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Year: 2011 PMID： 21851837 DOI： 10.1016/j.mimet.2011.07.018

Source DB: PubMed Journal: J Microbiol Methods ISSN： 0167-7012 Impact factor: 2.363

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Cited

8 in total

1. Simple and efficient recycling of fungal selectable marker genes with the Cre-loxP recombination system via anastomosis.

Authors: Dong-Xiu Zhang; Hsiao-Ling Lu; Xinggang Liao; Raymond J St Leger; Donald L Nuss
Journal: Fungal Genet Biol Date: 2013-09-03 Impact factor: 3.495

2. Metabolic engineering of an industrial Aspergillus niger strain for itaconic acid production.

Authors: Hui Xie; Qinyuan Ma; Dongzhi Wei; Fengqing Wang
Journal: 3 Biotech Date: 2020-02-14 Impact factor: 2.406

3. The MrCYP52 cytochrome P450 monoxygenase gene of Metarhizium robertsii is important for utilizing insect epicuticular hydrocarbons.

Authors: Liangcai Lin; Weiguo Fang; Xinggang Liao; Fengqing Wang; Dongzhi Wei; Raymond J St Leger
Journal: PLoS One Date: 2011-12-16 Impact factor: 3.240

4. Metarhizium robertsii produces an extracellular invertase (MrINV) that plays a pivotal role in rhizospheric interactions and root colonization.

Authors: Xinggang Liao; Weiguo Fang; Liangcai Lin; Hsiao-Ling Lu; Raymond J St Leger
Journal: PLoS One Date: 2013-10-21 Impact factor: 3.240

5. Light-inducible genetic engineering and control of non-homologous end-joining in industrial eukaryotic microorganisms: LML 3.0 and OFN 1.0.

Authors: Lei Zhang; Xihua Zhao; Guoxiu Zhang; Jiajia Zhang; Xuedong Wang; Suping Zhang; Wei Wang; Dongzhi Wei
Journal: Sci Rep Date: 2016-02-09 Impact factor: 4.379

Chlorimuron ethyl as a new selectable marker for disrupting genes in the insect-pathogenic fungus Metarhizium robertsii.

1. Simple and efficient recycling of fungal selectable marker genes with the Cre-loxP recombination system via anastomosis.

2. Metabolic engineering of an industrial Aspergillus niger strain for itaconic acid production.

3. The MrCYP52 cytochrome P450 monoxygenase gene of Metarhizium robertsii is important for utilizing insect epicuticular hydrocarbons.

4. Metarhizium robertsii produces an extracellular invertase (MrINV) that plays a pivotal role in rhizospheric interactions and root colonization.

5. Light-inducible genetic engineering and control of non-homologous end-joining in industrial eukaryotic microorganisms: LML 3.0 and OFN 1.0.

6. Development of genetic tools for Myceliophthora thermophila.

7. Agrobacterium-Mediated Co-transformation of Multiple Genes in Metarhizium robertsii.

8. Enhanced UV resistance and improved killing of malaria mosquitoes by photolyase transgenic entomopathogenic fungi.