AIM: Lactobacillus plantarum AS1 was incubated with HT-29 adenocarcinoma cell line to assess its adhesion potency and examined for its inhibitory effect on the cell attachment by an enterovirulent bacterium Vibrio parahaemolyticus. METHODS AND RESULTS: Lactobacillus plantarum AS1 attached efficiently to HT-29 cells as revealed by scanning electron microscopy and bacterial adhesion assay. Lactobacillus plantarum AS1 significantly reduced V. parahaemolyticus attached to HT-29 cells by competition, exclusion and displacement mode. Lactobacillus plantarum AS1 seems to adhere to human intestinal cells via mechanisms that involve different combinations of carbohydrate and protein factors on the bacteria and eukaryotic cell surface. CONCLUSION: Strain Lact. plantarum AS1 inhibits the cell attachment of a pathogen V. parahaemolyticus by steric hindrance mechanism. Also, antibacterial factors such as bacteriocins, lactic acid and exopolysaccharides could be involved. SIGNIFICANCE AND IMPACT OF THE STUDY: The ability to inhibit the adhesion of V. parahaemolyticus to intestinal cell line warrants further investigation to explore the use of probiotic strain Lact. plantarum AS1 in the management of gastroenteritis caused by V. parahaemolyticus.
AIM: Lactobacillus plantarumAS1 was incubated with HT-29 adenocarcinoma cell line to assess its adhesion potency and examined for its inhibitory effect on the cell attachment by an enterovirulent bacterium Vibrio parahaemolyticus. METHODS AND RESULTS:Lactobacillus plantarumAS1 attached efficiently to HT-29 cells as revealed by scanning electron microscopy and bacterial adhesion assay. Lactobacillus plantarumAS1 significantly reduced V. parahaemolyticus attached to HT-29 cells by competition, exclusion and displacement mode. Lactobacillus plantarumAS1 seems to adhere to human intestinal cells via mechanisms that involve different combinations of carbohydrate and protein factors on the bacteria and eukaryotic cell surface. CONCLUSION: Strain Lact. plantarumAS1 inhibits the cell attachment of a pathogen V. parahaemolyticus by steric hindrance mechanism. Also, antibacterial factors such as bacteriocins, lactic acid and exopolysaccharides could be involved. SIGNIFICANCE AND IMPACT OF THE STUDY: The ability to inhibit the adhesion of V. parahaemolyticus to intestinal cell line warrants further investigation to explore the use of probiotic strain Lact. plantarumAS1 in the management of gastroenteritis caused by V. parahaemolyticus.
Authors: Milica Živković; Marija S Miljković; Patricia Ruas-Madiedo; Milica B Markelić; Katarina Veljović; Maja Tolinački; Svetlana Soković; Aleksandra Korać; Nataša Golić Journal: Front Microbiol Date: 2016-03-09 Impact factor: 5.640
Authors: Sebastian Beltran; Cristian A Munoz-Bergmann; Ana Elola-Lopez; Javiera Quintana; Cristopher Segovia; Annette N Trombert Journal: Biol Res Date: 2016-01-07 Impact factor: 5.612
Authors: Katarina Veljović; Nikola Popović; Marija Miljković; Maja Tolinački; Amarela Terzić-Vidojević; Milan Kojić Journal: Front Microbiol Date: 2017-09-26 Impact factor: 5.640