[Comparison of intracorporal absorption of hypaconitine in Heishunpian decoction and its compound recipe decoction by ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry].

An ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF MS) method was developed for the determination of hypaconitine in the plasma of the rats administered with Heishunpian (HSP) decoction, Zhufu (ZF) decoction and Gancaofuzi (GF) decoction, separately. Hypaconitine and the internal standard were separated on a ZORBAX Extend-C18 column. The concentration-time curve of hypaconitine in the plasma was protracted, and pharmacokinetic parameters were calculated, then the pharmacokinetic comparison of hypaconitine between HSP, ZF and GF was carried out for the first time. The results of the methodological test showed that the plasma concentration of hypaconitine presented good linear relationship in the range of 0.02-10 ng/mL. The results of the sample determination showed that the absorptive degree of ñfrom ZF decoction in the plasma was lower than that from HSP decoction, while the absorptive degree of hypaconitine from GF decoction in plasma was higher than that from HSP decoction. It was deduced that some components in ZF decoction can restrain the absorption of hypaconitine in plasma, and some components in GF decoction may promote the absorption of hypaconitine in plasma, and the difference in drug effects between ZF decoction and GF decoction may derive from the different absorptive degrees of hypaconitine in plasma. The pharmacokinetic property of hypaconitine was proposed to be non-linear dynamics on the basis of the drug elimination half life (T(1/2)) and the area under the plasma concentration-time curve (AUC).