BACKGROUND: The metabolic syndrome (MetS) is a cluster of risk factors that are highly associated with increased risk for cardiovascular disease (CVD). Increased serum levels of plasminogen activator inhibitor-1 (PAI-1), interleukin-6 (IL-6) and IL-18 have been reported to be associated with CVD. Recently, IL-18 has been shown to be predictive for cardiovascular events in subjects with MetS. We have investigated the expression of PAI-1, IL-6 and IL-18 in subcutaneous adipose tissue (AT) of subjects with (n = 22) and without (n = 36) MetS. Furthermore, we have analysed the expression of IL-18 in monocyte-derived macrophages (MDMs) in an in vitro model of hyperglycaemia. METHODS: We studied the expression of PAI-1, IL-6 and IL-18 in biopsies of subcutaneous adipose tissue using Real-time PCR. After isolation and cultivation of MDMs, expression of IL-18 was determined by Real-time PCR. RESULTS: Expression of IL-18 was increased in subcutaneous AT of subjects with MetS (p < 0.05). Multivariate analysis revealed fasting plasma glucose to be the only MetS component being independently associated with expression of IL-18 in AT (p < 0.05). Exposure to hyperglycaemia, increased in expression of IL-18 in MDMs (p < 0.01). CONCLUSION: Our findings suggest that subjects with MetS have a particular inflammatory pattern in AT, possibly driven by fasting glucose. MDMs might - at least in part - be the cellular source of this increased expression. Together with recent reports, showing IL-18 to be predictive for cardiovascular events, our findings could provide the basis for further research of the role of IL-18 as a link and possible target in the association between MetS and atherosclerosis.
BACKGROUND: The metabolic syndrome (MetS) is a cluster of risk factors that are highly associated with increased risk for cardiovascular disease (CVD). Increased serum levels of plasminogen activator inhibitor-1 (PAI-1), interleukin-6 (IL-6) and IL-18 have been reported to be associated with CVD. Recently, IL-18 has been shown to be predictive for cardiovascular events in subjects with MetS. We have investigated the expression of PAI-1, IL-6 and IL-18 in subcutaneous adipose tissue (AT) of subjects with (n = 22) and without (n = 36) MetS. Furthermore, we have analysed the expression of IL-18 in monocyte-derived macrophages (MDMs) in an in vitro model of hyperglycaemia. METHODS: We studied the expression of PAI-1, IL-6 and IL-18 in biopsies of subcutaneous adipose tissue using Real-time PCR. After isolation and cultivation of MDMs, expression of IL-18 was determined by Real-time PCR. RESULTS: Expression of IL-18 was increased in subcutaneous AT of subjects with MetS (p < 0.05). Multivariate analysis revealed fasting plasma glucose to be the only MetS component being independently associated with expression of IL-18 in AT (p < 0.05). Exposure to hyperglycaemia, increased in expression of IL-18 in MDMs (p < 0.01). CONCLUSION: Our findings suggest that subjects with MetS have a particular inflammatory pattern in AT, possibly driven by fasting glucose. MDMs might - at least in part - be the cellular source of this increased expression. Together with recent reports, showing IL-18 to be predictive for cardiovascular events, our findings could provide the basis for further research of the role of IL-18 as a link and possible target in the association between MetS and atherosclerosis.
Authors: Elsa M Hjerkinn; Michael Abdelnoor; Liv Breivik; Lise Bergengen; Ingrid Ellingsen; Ingebjørg Seljeflot; Ottar Aase; Tor Ole Klemsdal; Ingvar Hjermann; Harald Arnesen Journal: Eur J Cardiovasc Prev Rehabil Date: 2006-06
Authors: Stefan P Kastl; Walter S Speidl; Katharina M Katsaros; Christoph Kaun; Gersina Rega; Afshin Assadian; Georg W Hagmueller; Martina Hoeth; Rainer de Martin; Yongsheng Ma; Gerald Maurer; Kurt Huber; Johann Wojta Journal: Blood Date: 2009-08-03 Impact factor: 22.113
Authors: Vanesa Martínez-Barquero; Griselda de Marco; Sergio Martínez-Hervas; Victoria Adam-Felici; Cristina Pérez-Soriano; Verónica Gonzalez-Albert; Gemma Rojo; Juan Francisco Ascaso; José Tomás Real; Ana Barbara Garcia-Garcia; Juan Carlos Martín-Escudero; Raquel Cortes; Felipe Javier Chaves Journal: BMJ Open Date: 2017-11-15 Impact factor: 2.692