| Literature DB >> 21839139 |
Julie T Millard1, Erin E McGowan, Sharonda Q Bradley.
Abstract
The bifunctional alkylating agent 1,2,3,4-diepoxybutane (DEB) is thought to be a major contributor to the carcinogenicity of 1,3-butadiene, from which it is derived in vivo. DEB forms DNA interstrand cross-links primarily between distal deoxyguanosine residues at the duplex sequence 5'-GNC. In order for the short butanediol tether to span this distance, distortion of the DNA target has been postulated. We determined that the electrophoretic mobility of ligated DNA oligomers containing DEB cross-links was retarded in comparison with control, uncross-linked DNA. Our data are consistent with DNA bending of ∼34° per lesion towards the major groove.Entities:
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Year: 2011 PMID： 21839139 PMCID： PMC3236281 DOI： 10.1016/j.biochi.2011.07.030
Source DB: PubMed Journal: Biochimie ISSN： 0300-9084 Impact factor: 4.079