Literature DB >> 2183794

Evidence for presence of peptide alpha-amidating activity in pancreatic islets from newborn rats.

A Zhou1, N A Thorn.   

Abstract

The peptide alpha-amidating activity of a homogenate of pancreatic islets from 5-7-day-old rats was investigated, using as substrate a glycine-extended tripeptide (D-Tyr-Val-Gly). The islet homogenates had a marked amidating activity, with a Km of 57 microM, a Vmax. of 185 pmol/h per mg and a pH optimum of 7.0. This activity was dependent on the presence of ascorbic acid (in the reduced form) and Cu2+, the optimum concentrations being 4 mM and 40 microM respectively. On fractionation of the homogenate, the highest specific activity was found in the soluble fraction. Exocrine pancreatic tissue showed very low levels of amidating activity.

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Year:  1990        PMID： 2183794      PMCID： PMC1131273          DOI： 10.1042/bj2670253

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  14 in total

1.  Evidence for high peptide alpha-amidation activity in the neonatal rat pancreas.

Authors:  L Ouafik; A Dutour; P Salers; P Giraud; F Boudouresque; E Castanas; C Oliver
Journal:  Biochem Biophys Res Commun       Date:  1986-07-16       Impact factor: 3.575

2.  Fluorometric assay of proteins in the nanogram range.

Authors:  P Böhlen; S Stein; W Dairman; S Udenfriend
Journal:  Arch Biochem Biophys       Date:  1973-03       Impact factor: 4.013

3.  A rapid, sensitive assay for glycine-directed amidating enzymes.

Authors:  L J Moray; C R Miller; W H Busby; J Humm; R C Bateman; J S Kizer
Journal:  J Neurosci Methods       Date:  1985-09       Impact factor: 2.390

4.  Identification in pituitary tissue of a peptide alpha-amidation activity that acts on glycine-extended peptides and requires molecular oxygen, copper, and ascorbic acid.

Authors:  B A Eipper; R E Mains; C C Glembotski
Journal:  Proc Natl Acad Sci U S A       Date:  1983-08       Impact factor: 11.205

5.  Bovine intermediate pituitary alpha-amidation enzyme: preliminary characterization.

Authors:  B A Eipper; C C Glembotski; R E Mains
Journal:  Peptides       Date:  1983 Nov-Dec       Impact factor: 3.750

6.  Secretion and regulation of two biosynthetic enzyme activities, peptidyl-glycine alpha-amidating monooxygenase and a carboxypeptidase, by mouse pituitary corticotropic tumor cells.

Authors:  R E Mains; B A Eipper
Journal:  Endocrinology       Date:  1984-11       Impact factor: 4.736

7.  Purification and characterization of peptidylglycine alpha-amidating monooxygenase from bovine neurointermediate pituitary.

Authors:  A S Murthy; R E Mains; B A Eipper
Journal:  J Biol Chem       Date:  1986-02-05       Impact factor: 5.157

8.  Structure of the precursor to an enzyme mediating COOH-terminal amidation in peptide biosynthesis.

Authors:  B A Eipper; L P Park; I M Dickerson; H T Keutmann; E A Thiele; H Rodriguez; P R Schofield; R E Mains
Journal:  Mol Endocrinol       Date:  1987-11

9.  Hormonal, drug, and dietary factors affecting peptidyl glycine alpha-amidating monooxygenase activity in various tissues of the adult male rat.

Authors:  R E Mains; A C Myers; B A Eipper
Journal:  Endocrinology       Date:  1985-06       Impact factor: 4.736

10.  Altered amidation of pancreatic polypeptide in cultured dog islet tissue.

Authors:  T L Paquette; R Gingerich; D Scharp
Journal:  Biochemistry       Date:  1981-12-22       Impact factor: 3.162
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  5 in total

1.  Transport of ascorbic acid and dehydroascorbic acid by pancreatic islet cells from neonatal rats.

Authors:  A Zhou; J H Nielsen; O Farver; N A Thorn
Journal:  Biochem J       Date:  1991-03-15       Impact factor: 3.857

2.  Distribution of peptidyl-glycine alpha-amidating monooxygenase immunoreactivity in the brain, pituitary and islet organ of the anglerfish (Lophius americanus).

Authors:  J K McDonald; K Klein; B D Noe
Journal:  Cell Tissue Res       Date:  1995-04       Impact factor: 5.249

Review 3.  Insights into pancreatic islet cell dysfunction from type 2 diabetes mellitus genetics.

Authors:  Nicole A J Krentz; Anna L Gloyn
Journal:  Nat Rev Endocrinol       Date:  2020-02-25       Impact factor: 43.330

4.  High ascorbic acid content in the rat endocrine pancreas.

Authors:  A Zhou; N A Thorn
Journal:  Diabetologia       Date:  1991-11       Impact factor: 10.122

5.  Type 2 diabetes risk alleles in PAM impact insulin release from human pancreatic β-cells.

Authors:  Soren K Thomsen; Anne Raimondo; Benoit Hastoy; Shahana Sengupta; Xiao-Qing Dai; Austin Bautista; Jenny Censin; Anthony J Payne; Mahesh M Umapathysivam; Aliya F Spigelman; Amy Barrett; Christopher J Groves; Nicola L Beer; Jocelyn E Manning Fox; Mark I McCarthy; Anne Clark; Anubha Mahajan; Patrik Rorsman; Patrick E MacDonald; Anna L Gloyn
Journal:  Nat Genet       Date:  2018-07-27       Impact factor: 38.330
  5 in total

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