INTRODUCTION: An automated method is described for the rapid and high-yield synthesis of two of the most commonly used radioactive fatty acids: [(11)C]acetate and [(11)C]palmitate. METHODS: Reaction of [(11)C]CO(2) with the respective Grignard reagents in diethyl ether solution proceeded for 2 min at 40°C. Quenching of the reaction and liberation of nonreacted [(11)C]CO(2) occurred upon addition of a fourfold molar excess of aqueous 0.1 M HCl (acetate) or nonaqueous HCl/Et(2)O (palmitate). Labeled products were then purified by adsorption to an Alumina-N Sep-Pak Plus cartridge and eluted with either aqueous NaH(2)PO(4) solution (acetate) or 100% ethanol (palmitate). RESULTS: High-performance liquid chromatography analysis confirmed that the radiochemical purity of each product was >98%, and decay-corrected radiochemical yields averaged 33% for [(11)C]palmitate and 40% for [(11)C]acetate. CONCLUSION: The method requires no liquid-liquid extraction, solvent evaporation or distillation capabilities and can be readily adapted to existing radiosynthesis modules.
INTRODUCTION: An automated method is described for the rapid and high-yield synthesis of two of the most commonly used radioactive fatty acids: [(11)C]acetate and [(11)C]palmitate. METHODS: Reaction of [(11)C]CO(2) with the respective Grignard reagents in diethyl ether solution proceeded for 2 min at 40°C. Quenching of the reaction and liberation of nonreacted [(11)C]CO(2) occurred upon addition of a fourfold molar excess of aqueous 0.1 M HCl (acetate) or nonaqueous HCl/Et(2)O (palmitate). Labeled products were then purified by adsorption to an Alumina-N Sep-Pak Plus cartridge and eluted with either aqueous NaH(2)PO(4) solution (acetate) or 100% ethanol (palmitate). RESULTS: High-performance liquid chromatography analysis confirmed that the radiochemical purity of each product was >98%, and decay-corrected radiochemical yields averaged 33% for [(11)C]palmitate and 40% for [(11)C]acetate. CONCLUSION: The method requires no liquid-liquid extraction, solvent evaporation or distillation capabilities and can be readily adapted to existing radiosynthesis modules.
Authors: Yinlin Li; Tao Huang; Xinyue Zhang; Min Zhong; Natalie N Walker; Jiang He; Stuart S Berr; Susanna R Keller; Bijoy K Kundu Journal: Mol Imaging Date: 2015 Impact factor: 4.488
Authors: Yen Ng; Steven P Moberly; Kieren J Mather; Clive Brown-Proctor; Gary D Hutchins; Mark A Green Journal: Nucl Med Biol Date: 2013-01-08 Impact factor: 2.408
Authors: Paul R Territo; Mary Maluccio; Amanda A Riley; Brian P McCarthy; James Fletcher; Mark Tann; Romil Saxena; Nicholas J Skill Journal: BMC Med Imaging Date: 2015-05-16 Impact factor: 1.930
Authors: Nana L Christensen; Steen Jakobsen; Anna C Schacht; Ole L Munk; Aage K O Alstrup; Lars P Tolbod; Hendrik J Harms; Søren Nielsen; Lars C Gormsen Journal: Mol Imaging Date: 2017 Jan-Dec Impact factor: 4.488