Lei Geng1, Junfang Deng, Guoping Jiang, Penghong Song, Zhuoyi Wang, Zhijun Jiang, Min Zhang, Shusen Zheng. 1. Division of Hepatobiliary and Pancreatic Surgery, Department of Surgery, Key Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health, Zhejiang Province, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
Abstract
BACKGROUND/AIMS: Interleukin-10 (IL-10) plays an important role in hepatocellular carcinoma (HCC) immune evasion. However, the precise mechanism responsible for aberrant IL-10 production remains unknown. B7-H1 expression can predominantly stimulate IL-10 production and contributes to the tumor immune evasion. This study was designed to investigate the expression of B7-H1 and IL-10 in normal liver tissues and HCC samples, and further to evaluate the correlation between B7-H1 expression and IL-10 levels. METHODOLOGY: We detected the B7-H1 and IL-10 expression in sixty HCC tissues and ten healthy liver tissues at mRNA and protein level using RT-PCR and immunohistochemical staining Western-blotting, respectively, and further analyzed the correlation between B7-H1 expression and IL-10 levels in HCC samples. RESULTS: The results showed that all HCC samples demonstrated the up-regulated expression of B7-H1 and IL-10 at mRNA and protein level compared with normal liver tissues and there was a significant correlation between B7-H1 expression and IL-10 levels (p<0.01). CONCLUSION: These findings for the first time suggest the potential role of B7-H1 in promoting IL-10 production in HCC tissue, and implicate a new mechanism of HCC escape from immune surveillance.
BACKGROUND/AIMS: Interleukin-10 (IL-10) plays an important role in hepatocellular carcinoma (HCC) immune evasion. However, the precise mechanism responsible for aberrant IL-10 production remains unknown. B7-H1 expression can predominantly stimulate IL-10 production and contributes to the tumor immune evasion. This study was designed to investigate the expression of B7-H1 and IL-10 in normal liver tissues and HCC samples, and further to evaluate the correlation between B7-H1 expression and IL-10 levels. METHODOLOGY: We detected the B7-H1 and IL-10 expression in sixty HCC tissues and ten healthy liver tissues at mRNA and protein level using RT-PCR and immunohistochemical staining Western-blotting, respectively, and further analyzed the correlation between B7-H1 expression and IL-10 levels in HCC samples. RESULTS: The results showed that all HCC samples demonstrated the up-regulated expression of B7-H1 and IL-10 at mRNA and protein level compared with normal liver tissues and there was a significant correlation between B7-H1 expression and IL-10 levels (p<0.01). CONCLUSION: These findings for the first time suggest the potential role of B7-H1 in promoting IL-10 production in HCC tissue, and implicate a new mechanism of HCC escape from immune surveillance.
Authors: Kostandinos Sideras; Katharina Biermann; Joanne Verheij; Bart R Takkenberg; Shanta Mancham; Bettina E Hansen; Hannah M Schutz; Robert A de Man; Dave Sprengers; Sonja I Buschow; Maddy C M Verseput; Patrick P C Boor; Qiuwei Pan; Thomas M van Gulik; Turkan Terkivatan; Jan N M Ijzermans; Ulrich H W Beuers; Stefan Sleijfer; Marco J Bruno; Jaap Kwekkeboom Journal: Oncoimmunology Date: 2017-01-03 Impact factor: 8.110
Authors: Kostandinos Sideras; Robert A de Man; Susan M Harrington; Wojciech G Polak; Guoying Zhou; Hannah M Schutz; Alexander Pedroza-Gonzalez; Katharina Biermann; Shanta Mancham; Bettina E Hansen; R Bart Takkenberg; Anneke J van Vuuren; Qiuwei Pan; Jan N M Ijzermans; Stefan Sleijfer; Dave Sprengers; Haidong Dong; Jaap Kwekkeboom; Marco J Bruno Journal: Sci Rep Date: 2019-07-23 Impact factor: 4.379
Authors: Pablo A González; Leandro J Carreño; Pablo F Céspedes; Susan M Bueno; Claudia A Riedel; Alexis M Kalergis Journal: Clin Dev Immunol Date: 2013-03-07