BACKGROUND: A defective innate immune response may contribute to the pathogenesis of Crohn's disease (CD) and ulcerative colitis (UC). Employing a global gene expression analysis, this study was aimed at identifying specifically regulated genes within the epithelial compartment in inflammatory bowel disease (IBD). METHODS: The epithelial fraction of human ileal mucosa samples from surgical specimens was obtained by laser microdissection. Gene expression was examined by global expression profiling (n = 18, Affymetrix), quantitative reverse-transcription polymerase chain reaction (RT-PCR) (n = 35), immunoblot analysis (n = 9), and immunohistochemistry (n = 25). RESULTS: Global expression profiling revealed a pronounced downregulation of the retinoic acid-inducible gene I (RIG-I) within the epithelial layer of the ileum in patients with CD but not with UC. The downregulation of RIG-I was confirmed by quantitative RT-PCR, immunoblot analysis, and immunohistochemistry. CONCLUSIONS: Epithelial downregulation of RIG-I, a known pattern recognition receptor for viral components, might contribute to alterations of the innate mucosal immune response, particularly in CD.
BACKGROUND: A defective innate immune response may contribute to the pathogenesis of Crohn's disease (CD) and ulcerative colitis (UC). Employing a global gene expression analysis, this study was aimed at identifying specifically regulated genes within the epithelial compartment in inflammatory bowel disease (IBD). METHODS: The epithelial fraction of human ileal mucosa samples from surgical specimens was obtained by laser microdissection. Gene expression was examined by global expression profiling (n = 18, Affymetrix), quantitative reverse-transcription polymerase chain reaction (RT-PCR) (n = 35), immunoblot analysis (n = 9), and immunohistochemistry (n = 25). RESULTS: Global expression profiling revealed a pronounced downregulation of the retinoic acid-inducible gene I (RIG-I) within the epithelial layer of the ileum in patients with CD but not with UC. The downregulation of RIG-I was confirmed by quantitative RT-PCR, immunoblot analysis, and immunohistochemistry. CONCLUSIONS: Epithelial downregulation of RIG-I, a known pattern recognition receptor for viral components, might contribute to alterations of the innate mucosal immune response, particularly in CD.
Authors: Anna E Russ; Jason S Peters; Warren C McNabb; Matthew P G Barnett; Rachel C Anderson; Zaneta Park; Shuotun Zhu; Paul Maclean; Wayne Young; Gordon W Reynolds; Nicole C Roy Journal: PLoS One Date: 2013-05-20 Impact factor: 3.240
Authors: Mara Cananzi; Elizabeth Wohler; Antonio Marzollo; Nara Lygia de Macena Sobreira; Helen C Su; Anthony L Guerrerio; Davide Colavito; Jing You; Huie Jing; Silvia Bresolin; Paola Gaio; Renan Martin; Claudia Mescoli; Sangeeta Bade; Jennifer E Posey; Maurizio Dalle Carbonare; Wesley Tung; Shalini N Jhangiani; Luca Bosa; Yu Zhang; Joselito Sobreira Filho; Maria Gabelli; Richard Kellermayer; Howard A Kader; Maria Oliva-Hemker; Giorgio Perilongo; James R Lupski; Alessandra Biffi; David Valle; Alberta Leon Journal: Hum Genet Date: 2021-06-29 Impact factor: 5.881
Authors: Rithvik R Nalamalapu; Michelle Yue; Aaron R Stone; Samantha Murphy; Margaret S Saha Journal: Front Mol Neurosci Date: 2021-06-28 Impact factor: 5.639