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Literature DB >> 2182676

Culture confirmation of Escherichia coli serotype O157:H7 by direct immunofluorescence.

Abstract

An evaluation of a fluorescein-labeled, polyclonal, affinity-purified goat antibody to Escherichia coli serotype O157:H7 (Kirkegaard & Perry Laboratories Inc., Gaithersburg, Md.) was conducted to determine the efficacy of this research reagent for the rapid direct immunofluorescence identification of E. coli O157:H7 isolated from fecal specimens cultured on sorbitol-MacConkey (SMAC) agar. The E. coli O157:H7 fluorescent-antibody conjugate proved to be 100% sensitive and specific for the rapid identification of non-sorbitol-fermenting E. coli O157:H7 from SMAC agar inoculated with fecal specimens. The addition of SMAC agar to the battery of primary isolation media routinely used for fecal specimens resulted in the identification of 16 patients with E. coli O157:H7 disease from a total of 799 fecal specimens cultured during 1988.

Entities: Chemical Species

Mesh：

Year: 1990 PMID： 2182676 PMCID： PMC269675 DOI： 10.1128/jcm.28.3.612-613.1990

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

14 in total

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Authors: W R Gransden; M A Damm; J D Anderson; J E Carter; H Lior
Journal: J Infect Dis Date: 1986-09 Impact factor: 5.226

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Authors: S B March; S Ratnam
Journal: J Clin Microbiol Date: 1989-07 Impact factor: 5.948

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Journal: J Clin Microbiol Date: 1985-10 Impact factor: 5.948

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Journal: J Clin Microbiol Date: 1987-08 Impact factor: 5.948

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Authors: K L MacDonald; M J O'Leary; M L Cohen; P Norris; J G Wells; E Noll; J M Kobayashi; P A Blake
Journal: JAMA Date: 1988-06-24 Impact factor: 56.272

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Authors: A O O'Brien; T A Lively; M E Chen; S W Rothman; S B Formal
Journal: Lancet Date: 1983-03-26 Impact factor: 79.321

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Authors: M A Neill; P I Tarr; C R Clausen; D L Christie; R O Hickman
Journal: Pediatrics Date: 1987-07 Impact factor: 7.124

2. Humoral immune responses to Shiga-like toxins and Escherichia coli O157 lipopolysaccharide in hemolytic-uremic syndrome patients and healthy subjects.

Authors: J S Greatorex; G M Thorne
Journal: J Clin Microbiol Date: 1994-05 Impact factor: 5.948

3. Immunomagnetic-electrochemiluminescent detection of Escherichia coli O157 and Salmonella typhimurium in foods and environmental water samples.

Authors: H Yu; J G Bruno
Journal: Appl Environ Microbiol Date: 1996-02 Impact factor: 4.792

4. Monoclonal antibodies for detection of the H7 antigen of Escherichia coli.

Authors: Y He; J E Keen; R B Westerman; E T Littledike; J Kwang
Journal: Appl Environ Microbiol Date: 1996-09 Impact factor: 4.792

4 in total