Literature DB >> 2182382

Cloning of the genes encoding urease from Proteus vulgaris and sequencing of the structural genes.

G Mörsdorf1, H Kaltwasser.   

Abstract

A fragment of chromosomal DNA from proteus vulgaris encoding urease was cloned and expressed in Escherichia coli. A 3 kbp region was sequenced and revealed three open reading frames with homology to jack bean (Canavalia ensiformis) urease. The smallest protein (11 kDa) was homologous to the N-terminus of the plant enzyme and the largest polypeptide (61 kDa) corresponded to the C-terminus. The large protein contained conserved regions and a cysteine residue which is known to be catalytically important in the plant enzyme. A protein of 12 kDa showed homology to residues 132 to 237 of jack bean urease.

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Year:  1990        PMID： 2182382     DOI： 10.1016/0378-1097(90)90260-w

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN： 0378-1097            Impact factor:   2.742


  21 in total

1.  Sequence of the Klebsiella aerogenes urease genes and evidence for accessory proteins facilitating nickel incorporation.

Authors:  S B Mulrooney; R P Hausinger
Journal:  J Bacteriol       Date:  1990-10       Impact factor: 3.490

2.  Cytotoxicity of the HpmA hemolysin and urease of Proteus mirabilis and Proteus vulgaris against cultured human renal proximal tubular epithelial cells.

Authors:  H L Mobley; G R Chippendale; K G Swihart; R A Welch
Journal:  Infect Immun       Date:  1991-06       Impact factor: 3.441

3.  Klebsiella aerogenes urease gene cluster: sequence of ureD and demonstration that four accessory genes (ureD, ureE, ureF, and ureG) are involved in nickel metallocenter biosynthesis.

Authors:  M H Lee; S B Mulrooney; M J Renner; Y Markowicz; R P Hausinger
Journal:  J Bacteriol       Date:  1992-07       Impact factor: 3.490

4.  Urease from a potentially pathogenic coccoid isolate: purification, characterization, and comparison to other microbial ureases.

Authors:  S G Lee; D H Calhoun
Journal:  Infect Immun       Date:  1997-10       Impact factor: 3.441

Review 5.  Potential virulence factors of Proteus bacilli.

Authors:  A Rózalski; Z Sidorczyk; K Kotełko
Journal:  Microbiol Mol Biol Rev       Date:  1997-03       Impact factor: 11.056

6.  Characterization of a plasmid-encoded urease gene cluster found in members of the family Enterobacteriaceae.

Authors:  S E D'Orazio; C M Collins
Journal:  J Bacteriol       Date:  1993-03       Impact factor: 3.490

7.  The putative arthritogenic cationic 19-kilodalton antigen of Yersinia enterocolitica is a urease beta-subunit.

Authors:  M Skurnik; S Batsford; A Mertz; E Schiltz; P Toivanen
Journal:  Infect Immun       Date:  1993-06       Impact factor: 3.441

8.  Urease from Staphylococcus saprophyticus: purification, characterization and comparison to Staphylococcus xylosus urease.

Authors:  U K Schäfer; H Kaltwasser
Journal:  Arch Microbiol       Date:  1994       Impact factor: 2.552

9.  Threonine is present instead of cysteine at the active site of urease from Staphylococcus xylosus.

Authors:  J Jose; U K Schäfer; H Kaltwasser
Journal:  Arch Microbiol       Date:  1994       Impact factor: 2.552

10.  Site-directed mutagenesis of Klebsiella aerogenes urease: identification of histidine residues that appear to function in nickel ligation, substrate binding, and catalysis.

Authors:  I S Park; R P Hausinger
Journal:  Protein Sci       Date:  1993-06       Impact factor: 6.725
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