Hong-Ping Chen1, Jie-Hong Zhang, Xiao-Yu Wang, Lin Chen. 1. Key Lab of Chinese Medicinal Standardization of Ministry of Education, Chengdu University of TCM, Chengdu 610075, China. chen_hongping@126.com
Abstract
OBJECTIVE: To research the effect of processing with soils on the contents of Atractylode I, II, III of Rhizoma Atractylodis Macrocephalae. METHODS: Contents of Atractylode I, II, III were determined by HPLC. Hypersil-ODS C18 (200 mm x 4.6 mm, 5 microm) column was used; The mobile phase consisted of acetonitrile-water; The flow rate was 1.0 mL/min; The detection wavelength was 220 nm and 276 nm,the column temperature was 30 degrees C. RESULTS: All the indexes of Atractylode I, II, III peak were qualified; The result of methodological study was met the requirement of HPLC; The average contents of Atractylode I , II , II in raw Rhizoma Atractylodis Macrocephalae were 0.4365, 0.2878, 0.4140 mg/g, the soil-processed Rhizoma Atractylodis Macrocephalae were 0.5503, 0.3013, 0.8403 mg/g, the processed Rhizoma Atractylodis Macrocephalae were 0.5386, 0.2958, 0.7399 mg/g. CONCLUSION: The content of Atractylode II is no significant difference among the raw Rhizoma Atractylodis Macrocephalae and the Processed. The contents of both Atractylode I and III increased after processed. However, there is no significant difference between the soil-processed and the processed Rhizoma Atractylodis Macrocephalae with nothing.
OBJECTIVE: To research the effect of processing with soils on the contents of Atractylode I, II, III of Rhizoma Atractylodis Macrocephalae. METHODS: Contents of Atractylode I, II, III were determined by HPLC. Hypersil-ODS C18 (200 mm x 4.6 mm, 5 microm) column was used; The mobile phase consisted of acetonitrile-water; The flow rate was 1.0 mL/min; The detection wavelength was 220 nm and 276 nm,the column temperature was 30 degrees C. RESULTS: All the indexes of Atractylode I, II, III peak were qualified; The result of methodological study was met the requirement of HPLC; The average contents of Atractylode I , II , II in raw Rhizoma Atractylodis Macrocephalae were 0.4365, 0.2878, 0.4140 mg/g, the soil-processed Rhizoma Atractylodis Macrocephalae were 0.5503, 0.3013, 0.8403 mg/g, the processed Rhizoma Atractylodis Macrocephalae were 0.5386, 0.2958, 0.7399 mg/g. CONCLUSION: The content of Atractylode II is no significant difference among the raw Rhizoma Atractylodis Macrocephalae and the Processed. The contents of both Atractylode I and III increased after processed. However, there is no significant difference between the soil-processed and the processed Rhizoma Atractylodis Macrocephalae with nothing.