Literature DB >> 21822875

Classical cytogenetics: karyotyping techniques.

Steven E Bates1.   

Abstract

Classical cytogenetics by karyotyping has been utilized in clinical research laboratories for more than 50 years and remains the key method used in the stem cell laboratory to assess the genetic stability of stem cell cultures. It is currently the most readily accessible method for detecting chromosomal abnormalities in pluripotent stem cell cultures. This chapter will describe (1) how to prepare a culture to maximize the number of metaphase cells, (2) how to prepare slides containing chromosome spreads (3) methods used to stain chromosomes, and (4) how to interpret the cytogenetic report.

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Year:  2011        PMID： 21822875     DOI： 10.1007/978-1-61779-201-4_13

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  11 in total

1.  A simple, cost-effective but highly efficient system for deriving ventricular cardiomyocytes from human pluripotent stem cells.

Authors:  Zhihui Weng; Chi-Wing Kong; Lihuan Ren; Ioannis Karakikes; Lin Geng; Jiaozi He; Maggie Zi Ying Chow; Chong Fai Mok; Wendy Keung; Howard Chow; Anskar Y H Leung; Roger J Hajjar; Ronald A Li; Camie W Chan
Journal:  Stem Cells Dev       Date:  2014-04-22       Impact factor: 3.272

2.  Screening of human pluripotent stem cells using CGH and FISH reveals low-grade mosaic aneuploidy and a recurrent amplification of chromosome 1q.

Authors:  Michal Dekel-Naftali; Ayala Aviram-Goldring; Talia Litmanovitch; Jana Shamash; Haike Reznik-Wolf; Ilana Laevsky; Michal Amit; Joseph Itskovitz-Eldor; Yuval Yung; Ariel Hourvitz; Eyal Schiff; Shlomit Rienstein
Journal:  Eur J Hum Genet       Date:  2012-06-20       Impact factor: 4.246

3.  Detection of copy number variants using chromosomal microarray analysis for the prenatal diagnosis of congenital heart defects with normal karyotype.

Authors:  Tingting Song; Shanning Wan; Yu Li; Ying Xu; Yinghui Dang; Yunyun Zheng; Chunyan Li; Jiao Zheng; Biliang Chen; Jianfang Zhang
Journal:  J Clin Lab Anal       Date:  2018-07-25       Impact factor: 2.352

4.  Optical genome mapping enables constitutional chromosomal aberration detection.

Authors:  Tuomo Mantere; Kornelia Neveling; Céline Pebrel-Richard; Marion Benoist; Guillaume van der Zande; Ellen Kater-Baats; Imane Baatout; Ronald van Beek; Tony Yammine; Michiel Oorsprong; Faten Hsoumi; Daniel Olde-Weghuis; Wed Majdali; Susan Vermeulen; Marc Pauper; Aziza Lebbar; Marian Stevens-Kroef; Damien Sanlaville; Jean Michel Dupont; Dominique Smeets; Alexander Hoischen; Caroline Schluth-Bolard; Laïla El Khattabi
Journal:  Am J Hum Genet       Date:  2021-07-07       Impact factor: 11.025

5.  Translocation Breakpoints Preferentially Occur in Euchromatin and Acrocentric Chromosomes.

Authors:  Cheng-Yu Lin; Ankit Shukla; John P Grady; J Lynn Fink; Eloise Dray; Pascal H G Duijf
Journal:  Cancers (Basel)       Date:  2018-01-08       Impact factor: 6.639

Review 6.  Genetic testing for men with infertility: techniques and indications.

Authors:  Daniel L Pelzman; Kathleen Hwang
Journal:  Transl Androl Urol       Date:  2021-03

7.  Arrhythmic Risk Assessment of Hypokalaemia Using Human Pluripotent Stem Cell-Derived Cardiac Anisotropic Sheets.

Authors:  Bimal Gurung; Gary Tse; Wendy Keung; Ronald A Li; Wing Tak Wong
Journal:  Front Cell Dev Biol       Date:  2021-12-06

8.  Genetic analysis and clinical significance of a rare t(1;12)(q21;p13) in a patient with high-risk myelodysplastic syndrome.

Authors:  Fang Fang; Ru Jia; Congyan Liu; Hong Zhao; Wanling Sun
Journal:  Mol Genet Genomic Med       Date:  2022-02-22       Impact factor: 2.183

9.  Neuroinflammatory Markers in the Serum of Prepubertal Children with Down Syndrome.

Authors:  Luigi Tarani; Valentina Carito; Giampiero Ferraguti; Carla Petrella; Antonio Greco; Massimo Ralli; Marisa Patrizia Messina; Debora Rasio; Enrica De Luca; Carolina Putotto; Paolo Versacci; Mauro Ceccanti; Marco Fiore
Journal:  J Immunol Res       Date:  2020-03-23       Impact factor: 4.818

Review 10.  Chromosome Instability; Implications in Cancer Development, Progression, and Clinical Outcomes.

Authors:  Raghvendra Vishwakarma; Kirk J McManus
Journal:  Cancers (Basel)       Date:  2020-03-29       Impact factor: 6.639
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