Hai-Yan Yu1, Yi-Bo Luo, Wei-Ning Bai. 1. State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany,Chinese Academy of Sciences, Beijing 100093, China.
Abstract
PREMISE OF THE STUDY: Microsatellite markers were developed for Incarvillea sinensis var. sinensis (Bignoniaceae), an annual herb endemic to Inner Mongolia, to study the degree to which delayed self-fertilization is favored. METHODS AND RESULTS: Eight polymorphic primer sets were isolated and characterized in two Inner Mongolia populations of I. sinensis var. sinensis with a relatively simple and fast subcloning method. Numbers of alleles per locus ranged from 2 to 7, with observed and expected heterozygosities ranging from 0 to 0.261 and from 0 to 0.778, respectively. CONCLUSIONS: These markers will be useful for future studies of self-fertilization adaptability in I. sinensis var. sinensis.
PREMISE OF THE STUDY: Microsatellite markers were developed for Incarvillea sinensis var. sinensis (Bignoniaceae), an annual herb endemic to Inner Mongolia, to study the degree to which delayed self-fertilization is favored. METHODS AND RESULTS: Eight polymorphic primer sets were isolated and characterized in two Inner Mongolia populations of I. sinensis var. sinensis with a relatively simple and fast subcloning method. Numbers of alleles per locus ranged from 2 to 7, with observed and expected heterozygosities ranging from 0 to 0.261 and from 0 to 0.778, respectively. CONCLUSIONS: These markers will be useful for future studies of self-fertilization adaptability in I. sinensis var. sinensis.