Literature DB >> 21820015

Microbial DNA extraction from intestinal biopsies is improved by avoiding mechanical cell disruption.

Franck Carbonero1, Gerardo M Nava, Ann C Benefiel, Eugene Greenberg, H Rex Gaskins.   

Abstract

Currently, standard protocols for microbial DNA extraction from intestinal tissues do not exist. We assessed the efficiency of a commercial kit with and without mechanical disruption. Better quality DNA was obtained without mechanical disruption. Thus, it appears that bead-beating is not required for efficient microbial DNA extraction from intestinal biopsies.
Copyright © 2011 Elsevier B.V. All rights reserved.

Entities:  

Mesh：

Substances：

Year:  2011        PMID： 21820015      PMCID： PMC3168962          DOI： 10.1016/j.mimet.2011.07.014

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  17 in total

Review 1.  Ecological consequences of the phylogenetic and physiological diversities of acetogens.

Authors:  Harold L Drake; Kirsten Küsel; Carola Matthies
Journal:  Antonie Van Leeuwenhoek       Date:  2002-08       Impact factor: 2.271

2.  The linear PCR reaction: a simple and robust method for sequencing amplified rRNA genes.

Authors:  T M Embley
Journal:  Lett Appl Microbiol       Date:  1991-09       Impact factor: 2.858

3.  Isolation of DNA from bacterial samples of the human gastrointestinal tract.

Authors:  Erwin G Zoetendal; Hans G H J Heilig; Eline S Klaassens; Carien C G M Booijink; Michiel Kleerebezem; Hauke Smidt; Willem M de Vos
Journal:  Nat Protoc       Date:  2006       Impact factor: 13.491

4.  Abundance and diversity of mucosa-associated hydrogenotrophic microbes in the healthy human colon.

Authors:  Gerardo M Nava; Franck Carbonero; Jennifer A Croix; Eugene Greenberg; H Rex Gaskins
Journal:  ISME J       Date:  2011-07-14       Impact factor: 10.302

5.  The genome of the Gram-positive metal- and sulfate-reducing bacterium Desulfotomaculum reducens strain MI-1.

Authors:  Pilar Junier; Thomas Junier; Sheila Podell; David R Sims; John C Detter; Athanasios Lykidis; Cliff S Han; Nicholas S Wigginton; Terry Gaasterland; Rizlan Bernier-Latmani
Journal:  Environ Microbiol       Date:  2010-10       Impact factor: 5.491

6.  Development of oligonucleotide probes and PCR primers for detecting phylogenetic subgroups of sulfate-reducing bacteria.

Authors:  K Daly; R J Sharp; A J McCarthy
Journal:  Microbiology       Date:  2000-07       Impact factor: 2.777

7.  Isolation and identification of methanogen-specific DNA from blanket bog peat by PCR amplification and sequence analysis.

Authors:  B A Hales; C Edwards; D A Ritchie; G Hall; R W Pickup; J R Saunders
Journal:  Appl Environ Microbiol       Date:  1996-02       Impact factor: 4.792

8.  Host DNA can interfere with detection of Borrelia burgdorferi in skin biopsy specimens by PCR.

Authors:  F B Cogswell; C E Bantar; T G Hughes; Y Gu; M T Philipp
Journal:  J Clin Microbiol       Date:  1996-04       Impact factor: 5.948

9.  Biostructure of fecal microbiota in healthy subjects and patients with chronic idiopathic diarrhea.

Authors:  Alexander Swidsinski; Vera Loening-Baucke; Hans Verstraelen; Sylwia Osowska; Yvonne Doerffel
Journal:  Gastroenterology       Date:  2008-04-18       Impact factor: 22.682

10.  Diversity and abundance of sulfate-reducing microorganisms in the sulfate and methane zones of a marine sediment, Black Sea.

Authors:  Julie Leloup; Alexander Loy; Nina J Knab; Christian Borowski; Michael Wagner; Bo Barker Jørgensen
Journal:  Environ Microbiol       Date:  2007-01       Impact factor: 5.491
View more
  5 in total

1.  Method for preparing DNA from feces in guanidine thiocyanate solution affects 16S rRNA-based profiling of human microbiota diversity.

Authors:  Koji Hosomi; Harumi Ohno; Haruka Murakami; Yayoi Natsume-Kitatani; Kumpei Tanisawa; Soichiro Hirata; Hidehiko Suzuki; Takahiro Nagatake; Tomomi Nishino; Kenji Mizuguchi; Motohiko Miyachi; Jun Kunisawa
Journal:  Sci Rep       Date:  2017-06-28       Impact factor: 4.379

2.  DNA extract characterization process for microbial detection methods development and validation.

Authors:  Nathan D Olson; Jayne B Morrow
Journal:  BMC Res Notes       Date:  2012-12-03

Review 3.  Sampling Strategies for Three-Dimensional Spatial Community Structures in IBD Microbiota Research.

Authors:  Shaocun Zhang; Xiaocang Cao; He Huang
Journal:  Front Cell Infect Microbiol       Date:  2017-02-24       Impact factor: 5.293

4.  Optimized bacterial DNA isolation method for microbiome analysis of human tissues.

Authors:  Carlijn E Bruggeling; Daniel R Garza; Soumia Achouiti; Wouter Mes; Bas E Dutilh; Annemarie Boleij
Journal:  Microbiologyopen       Date:  2021-06       Impact factor: 3.139

5.  A protocol for the subcellular fractionation of Saccharomyces cerevisiae using nitrogen cavitation and density gradient centrifugation.

Authors:  Yuchong Wang; Kathryn S Lilley; Stephen G Oliver
Journal:  Yeast       Date:  2014-02-20       Impact factor: 3.239
  5 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.