C Pinot1, A Deredjian, S Nazaret, E Brothier, B Cournoyer, C Segonds, S Favre-Bonté. 1. Université de Lyon, France Research Group on 'Bacterial Opportunistic Pathogens and Environment', UMR 5557 Ecologie Microbienne, CNRS, VetAgro Sup and Université Lyon 1, 43 Boulevard du 11 Novembre 1918, 69622 Villeurbanne Cedex, France.
Abstract
AIMS: Aim of the study is to identify accurately Stenotrophomonas maltophilia isolates recovered from environmental and clinical samples. METHODS AND RESULTS: Recovery of Sten. maltophilia-like isolates from soil samples using the vancomycin, imipenem, amphotericin B (VIA) selective agar medium enabled distinction of various morphotype colonies. A set of soil and clinical isolates was tested for species identification using different methods. 16S rDNA analyses showed the dark green with a blue halo morphotype to be typical Sten. maltophilia strains. The API-20NE, Vitek-2 and Biolog phenotypic analyses typically used for the identification of clinical isolates did not perform well on these soil isolates. The species-specific PCR screening targeting Sten. maltophilia 23S rDNA and the multiplex smeD/ggpS PCR, differentiating Sten. maltophilia from Stenotrophomonas rhizophila, were tested for improvement of these identification schemes. The latter multiplex PCR identified all isolates tested in this study, whatever be their origin. CONCLUSIONS: Isolation on VIA medium and confirmation of Sten. maltophilia species membership by smeD PCR is proposed to identify environmental and clinical isolates of Sten. maltophilia. SIGNIFICANCE AND IMPACT OF THE STUDY: The proposed approach enables isolation and identification of Sten. maltophilia from different environments in an easy and rapid way. This approach will be useful to accurately manage studies on the abundance and distribution of Sten. maltophilia in hospital and nonhospital environments.
AIMS: Aim of the study is to identify accurately Stenotrophomonas maltophilia isolates recovered from environmental and clinical samples. METHODS AND RESULTS: Recovery of Sten. maltophilia-like isolates from soil samples using the vancomycin, imipenem, amphotericin B (VIA) selective agar medium enabled distinction of various morphotype colonies. A set of soil and clinical isolates was tested for species identification using different methods. 16S rDNA analyses showed the dark green with a blue halo morphotype to be typical Sten. maltophilia strains. The API-20NE, Vitek-2 and Biolog phenotypic analyses typically used for the identification of clinical isolates did not perform well on these soil isolates. The species-specific PCR screening targeting Sten. maltophilia 23S rDNA and the multiplex smeD/ggpS PCR, differentiating Sten. maltophilia from Stenotrophomonas rhizophila, were tested for improvement of these identification schemes. The latter multiplex PCR identified all isolates tested in this study, whatever be their origin. CONCLUSIONS: Isolation on VIA medium and confirmation of Sten. maltophilia species membership by smeD PCR is proposed to identify environmental and clinical isolates of Sten. maltophilia. SIGNIFICANCE AND IMPACT OF THE STUDY: The proposed approach enables isolation and identification of Sten. maltophilia from different environments in an easy and rapid way. This approach will be useful to accurately manage studies on the abundance and distribution of Sten. maltophilia in hospital and nonhospital environments.
Authors: Newton T Okuno; Isabela R Freire; Renata T R S Segundo; Cristiane R Silva; Victor A Marin Journal: Curr Microbiol Date: 2018-08-28 Impact factor: 2.188
Authors: Benjamin Youenou; Edmond Hien; Amélie Deredjian; Elisabeth Brothier; Sabine Favre-Bonté; Sylvie Nazaret Journal: Environ Sci Pollut Res Int Date: 2016-09-30 Impact factor: 4.223
Authors: N Hansen; A K I Rasmussen; M J Fiandaca; K N Kragh; T Bjarnsholt; N Høiby; H Stender; L Guardabassi Journal: New Microbes New Infect Date: 2014-03-25